Low levels of nitric oxide (NO) in systemic sclerosis: inducible NO synthase production is decreased in cultured peripheral blood monocyte/macrophage cells
| Autor: | M. Levacher, André Kahan, A. Hernvann, P. Hilliquin, Yannick Allanore, Didier Borderie, Hervé Lemaréchal, Ohvanesse G. Ekindjian |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male medicine.medical_specialty Necrosis Fluorescent Antibody Technique Nitric Oxide Synthase Type II Antineoplastic Agents Inflammation Nitric Oxide Peripheral blood mononuclear cell Monocytes Nitric oxide Interferon-gamma chemistry.chemical_compound Rheumatology Internal medicine Blood plasma Humans Medicine Pharmacology (medical) Cells Cultured Nitrites Aged Nitrates Scleroderma Systemic biology Receptors IgE Tumor Necrosis Factor-alpha business.industry Macrophages Interleukin Middle Aged Flow Cytometry Nitric oxide synthase Endothelial stem cell Endocrinology chemistry Immunology biology.protein Female Interleukin-4 Nitric Oxide Synthase medicine.symptom business Interleukin-1 |
| Zdroj: | Rheumatology. 40:1089-1096 |
| ISSN: | 1462-0332 1462-0324 |
| Popis: | Objective. To investigate nitric oxide (NO) production and inducible NO synthase expression by cultured peripheral blood mononuclear cells (PBMC) in patients with systemic sclerosis (SSc). Methods. Eighteen patients with SSc were compared with two control groups: 16 patients with rheumatoid arthritis (RA) and 23 patients with mechanical sciatica. Nitrate was determined by fluorimetry in plasma and by spectrophotometry in supernatants. Inducible NO synthase (iNOS) was detected in cultured PBMC by immunofluorescence, immunoblotting and flow cytometry with or without treatment of the cells with interleukin (IL) 1b+ tumour necrosis factor a (TNF-a), IL-4 or interferon c (IFN-c) from day 1 to day 5. Results. NO metabolite concentrations were lower in SSc patients (mean " S.E.M. 34.3 " 2.63 mmolul) than in RA (48.3 " 2.82 mmolul; P< 0.02) and sciatica (43.3 " 5.24 mmolul; P< 0.03) patients. iNOS was detected in cultured monocytes in all three groups but induction occurred on day 1 in RA, day 2 in sciatica and only on day 3 in SSc, whatever the stimulus. Conclusions. The concentrations of NO metabolites are decreased in SSc patients and the metabolism of these compounds in PBMC is altered. Low levels of NO, a vasodilator, may be involved in vasospasm, which is critical in SSc. This may have therapeutic implications. |
| Databáze: | OpenAIRE |
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