In the quest of the optimal tissue source (porcine male and female articular, tracheal and auricular cartilage) for the development of collagen sponges for articular cartilage
| Autor: | Anne Maria Mullen, Z Wu, Athina Tzora, Dimitrios I. Zeugolis, Stefanie Korntner, Ioannis Skoufos |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Pore size
Auricular cartilage Adipose-derived stem cells Pathology medicine.medical_specialty biology Chemistry Cartilage Chondrogenic induction Cartilage engineering Articular cartilage General Medicine Chondrogenesis Staining medicine.anatomical_structure Pepsin Collagen type II Biological property medicine biology.protein Medical technology R855-855.5 |
| Zdroj: | Biomedical Engineering Advances, Vol 1, Iss, Pp 100002-(2021) |
| ISSN: | 2667-0992 |
| Popis: | Background: In the quest of the ideal material for scaffold fabrication for cartilage engineering, the use of collagen type II has been advocated, as it is the major constituent of cartilage tissue. Considering though the high batch-to-batch variability of animal-derived products, it is essential to identify suitable collagen type II sources. Methods: Herein, we ventured to assess the influence of biological sex (male, female) and tissue (articular, tracheal, auricular) on the biophysical, biochemical and biological properties of pepsin extracted porcine collagen type II scaffolds. Results: Articular cartilage resulted in pure collagen type II preparations, whilst tracheal and auricular cartilage preparations were contaminated with collagen type I. Pore size, porosity and denaturation temperature were not affected (p > 0.05) as a function of tissue and biological sex. Articular cartilage derived sponges exhibited significantly (p 0.05) differences between the groups in cell viability, DNA concentration, metabolic activity and Alcian blue staining. In general, the articular cartilage groups induced the highest (p |
| Databáze: | OpenAIRE |
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