On the clustered exchanges of the RecBCD pathway operating on phage lambda
| Autor: | Claire E. Shurvinton, Lynn C. Thomason, Mary M. Stahl, Franklin W. Stahl, Stuart A. Hill |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Genetic Markers
Exodeoxyribonuclease V Cointegrate Genotype Genes vpr Investigations Lambda chemistry.chemical_compound Plasmid Sequence Homology Nucleic Acid Genetics Centrifugation Density Gradient Escherichia coli RecBCD Recombination Genetic Endodeoxyribonucleases biology Plasmid recombination Lambda phage biology.organism_classification Molecular biology Bacteriophage lambda Exodeoxyribonucleases chemistry Lytic cycle Mutation Recombination Plasmids |
| Zdroj: | Genetics. 139(3) |
| ISSN: | 0016-6731 |
| Popis: | Lytic cycle crosses of Red- Gam- phage lambda were conducted in rec+ Escherichia coli carrying one or another plasmid with homology to lambda. Lambda x lambda recombinants and lambda x plasmid recombinants were formed by RecBCD-mediated recombination. We showed previously that the act of recombining with a plasmid alters the disposition of selected lambda x lambda exchanges. This work reports that the relationships between the lambda x plasmid and the lambda x lambda exchanges is unaltered by the removal from one lambda parent of the homology shared with the plasmid. This result supports our view that a reciprocal exchange, allowing for cointegrate formation, is associated with but mechanistically separable from a (presumably) nonreciprocal lambda x lambda exchange. The nature of this relationship is independent of lambda's Rap function, which is shown to alter the ratio of cointegrate formation (splices) to marker pick-up (patches) in lambda x plasmid recombination mediated by the RecBCD pathway. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|