Efficiency of various cloned DNA probes for detection of bovine viral diarrhea viruses
| Autor: | S. R. Bolin, E. Travis Littledike, Marc S. Collett, Jimmy Kwang |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Immunoblotting
Dot blot Microbiology Article Virus chemistry.chemical_compound Cytopathogenic Effect Viral Complementary DNA Animals Cells Cultured Diarrhea Viruses Bovine Viral General Veterinary biology Hybridization probe Pestivirus Nucleic Acid Hybridization General Medicine biology.organism_classification Virology Molecular biology chemistry DNA Viral Togaviridae Cattle DNA Probes Molecular probe DNA |
| Zdroj: | Veterinary Microbiology |
| ISSN: | 0378-1135 |
| Popis: | We have evaluated 24 cytopathic (CP) and 37 noncytopathic (NCP) strains of bovine viral diarrhea virus (BVDV) with a dot blot assay using four different genome segments of the NADL strain as hybridization probes (p80, p54, gp53, and gp62). The p80 and p54 probes hybridized to 23/24 (96%) and 22 24 (92%), respectively, of CP strains examined. In contrast, these same two probes only detected 16 36 (43%) and 5 36 (13%), respectively, of the NCP strains examined. The gp53 probe detected 18 24 (75%) and the gp62 probe detected 19 24 (79%) of the CP strains. In contrast, these latter two probes only detected 9 37 (24%) and 7 37 (20%), respectively, of NCP strains. This low detecteion rate of NCP strains suggests a need for developing a probe based on NCP sequences for identification of NCP strains. |
| Databáze: | OpenAIRE |
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