Multi-tissue patterning drives anterior morphogenesis of the C. elegans embryo
| Autor: | Karina Mastronardi, David S. Fay, Christopher Law, Ryan Christensen, Alisa Piekny, Stephanie Grimbert |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
biology ved/biology 030302 biochemistry & molecular biology ved/biology.organism_classification_rank.species Morphogenesis Embryo biology.organism_classification Cell biology 03 medical and health sciences Neuroblast Embryonic morphogenesis Model organism Process (anatomy) Caenorhabditis elegans 030304 developmental biology Lumen (unit) |
| DOI: | 10.1101/2020.04.27.064469 |
| Popis: | Complex structures derived from multiple tissue types are challenging to studyin vivo, and our knowledge of how cells from different tissues are coordinated is limited. Model organisms have proven invaluable for improving our understanding of how chemical and mechanical cues between cells from two different tissues can govern specific morphogenetic events. Here we usedCaenorhabditis elegansas a model system to show how cells from three different tissues are coordinated to give rise to the anterior lumen. This poorly understood process has remained a black box for embryonic morphogenesis. Using various microscopy and software approaches, we describe the movements and patterns of epidermal cells, neuroblasts and pharyngeal cells that contribute to lumen formation. The anterior-most pharyngeal cells (arcade cells) may provide the first marker for the location of the future lumen and facilitate the patterning of the surrounding neuroblasts. These neuroblast patterns control the rate of migration of the anterior epidermal cells, whereas the epidermal cells ultimately reinforce and control the position of the future lumen, as they must join with the pharyngeal cells for their epithelialization. Our studies are the first to characterize anterior morphogenesis inC. elegansin detail and should lay the framework for identifying how these different patterns are controlled at the molecular level. |
| Databáze: | OpenAIRE |
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