The Middle Subunit of Replication Protein A Contacts Growing RNA-DNA Primers in Replicating Simian Virus 40 Chromosomes
| Autor: | Gabriel Kaufmann, Gilad Mass, Tamar Nethanel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
DNA Replication
DNA clamp biology Errata DNA polymerase Ultraviolet Rays DNA polymerase II DNA replication Eukaryotic DNA replication Cell Biology DNA Primase Photoaffinity Labels Simian virus 40 Molecular biology DNA Dynamics and Chromosome Structure DNA-Binding Proteins Cross-Linking Reagents Control of chromosome duplication Replication Protein A DNA Viral biology.protein RNA Primase Molecular Biology Replication protein A |
| Popis: | The eukaryotic single-stranded DNA binding protein replication protein A (RPA) participates in major DNA transactions. RPA also interacts through its middle subunit (Rpa2) with regulators of the cell division cycle and of the response to DNA damage. A specific contact between Rpa2 and nascent simian virus 40 DNA was revealed by in situ UV cross-linking. The dynamic attributes of the cross-linked DNA, its size distribution, its RNA primer content, and its replication fork polarity were determined [corrected]. These data suggest that Rpa2 contacts the early DNA chain intermediates synthesized by DNA polymerase alpha-primase (RNA-DNA primers) but not more advanced products. Possible signaling functions of Rpa2 are discussed, and current models of eukaryotic lagging-strand DNA synthesis are evaluated in view of our results. |
| Databáze: | OpenAIRE |
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