Membrane biochemistry of the ouabain-resistant potassium transport system
| Autor: | L H English, Lewis C. Cantley, B White |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
ATPase
Sodium Drug Resistance chemistry.chemical_element Biological Transport Active Transfection Ouabain Cell Line Amiloride Mice Adenosine Triphosphate Chlorocebus aethiops polycyclic compounds Internal Medicine medicine Animals Vanadate 4-Nitrophenylphosphatase biology Chemistry Cell Membrane Fibroblasts biology.organism_classification Rubidium Molecular biology Adenosine Phosphoric Monoester Hydrolases Biochemistry biology.protein Green monkey Potassium Vanadates medicine.drug |
| Zdroj: | Hypertension (Dallas, Tex. : 1979). 10(5 Pt 2) |
| ISSN: | 0194-911X |
| Popis: | A 6.5-kilobase fragment of genomic DNA from mutant mouse cells under ouabain selection pressure conferred ouabain resistance when transfected into ouabain-sensitive CV1 green monkey fibroblasts. Ouabain resistance was induced in the presence of 10 microM ouabain. Amiloride (500 microM) completely blocked ouabain-insensitive 86Rb+ uptake into these cells. Plasma membranes from these cells demonstrated little sodium-dependent adenosine triphosphatase (ATPase) activity but had potassium-dependent and ouabain-resistant p-nitrophenylphosphatase activity. Like Na+,K+-ATPase this activity was vanadate- and sodium-inhibitable. Also, like the Na+,K+-ATPase, sodium inhibition of the p-nitrophenylphosphatase was reversed by 10 microM adenosine 5'-triphosphate. |
| Databáze: | OpenAIRE |
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