Modulatory effect of NO on sodium currents in a neuroblastoma cell line: Aspects of cell specificity
| Autor: | Maria Alexandra Ribeiro, Henrique Cabral, Pedro Freire Costa |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Patch-Clamp Techniques
Voltage clamp Population Nitric Oxide Sodium Channels Statistics Nonparametric Membrane Potentials Nitric oxide Mice Neuroblastoma chemistry.chemical_compound Cell Line Tumor Animals Drug Interactions Nitric Oxide Donors education Ion channel education.field_of_study Dose-Response Relationship Drug Thimerosal General Neuroscience Sodium channel Preservatives Pharmaceutical Nitrosylation Dose-Response Relationship Radiation General Medicine Electric Stimulation Potassium channel Biochemistry chemistry Biophysics |
| Zdroj: | Neuroscience Research. 58:361-370 |
| ISSN: | 0168-0102 |
| Popis: | Nitric oxide (NO) participates in the regulation of many cell functions in the CNS, including modulation of ion channel function by direct changes in the channel protein structure, modulating permeability or gating kinetics. The mechanisms by which NO donors modulate sodium currents are protein and tissue specific. The present paper concerns sodium currents in the neuroblastoma N1E-115 cell line, applying whole-cell voltage clamp methods. Sodium currents were characterized in terms of the sensitivity to NO donors and the hydrophilic thiol oxidizer thimerosal. Parameters defining steady-state inactivation and activation, removal of inactivation and the voltage dependence of inactivation, were determined before and after thimerosal application. The results concerning the application of thimerosal showed blockade of the resting state, hyperpolarizing shifts of m ∞ and h ∞ curves, change in the voltage sensitivity and slower inactivating kinetics, τ hf and τ hs being affected in the same manner. The present results provide clear evidence for redox modulation of the sodium channel population in N1E-115 cells. Our results showed that the membrane-permeable alkylating agent (NEM) does not inhibit current reduction determined by thimerosal. We have reasons to suspect that the sodium channel population in N1E-115 cells differs in the proposed consensus sequence for nitrosylation or thimerosal cysteine oxidation. |
| Databáze: | OpenAIRE |
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