Postsynaptic zinc potentiation elicited by KCl depolarization at hippocampal mossy fiber synapses
| Autor: | Fernando D. S. Sampaio dos Aidos, Paulo J. Mendes, Carlos M. Matias, Fatima C. Bastos, Rosa M. Santos, M. Emília Quinta-Ferreira, Jose C Dionisio, Rosa M. Quinta-Ferreira, Sandra Lopes, Vanessa N. Corceiro |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Physiology Long-Term Potentiation Biophysics chemistry.chemical_element Zinc Neurotransmission Inhibitory postsynaptic potential Synaptic Transmission Membrane Potentials Potassium Chloride 03 medical and health sciences 0302 clinical medicine Postsynaptic potential Animals Rats Wistar Cells Cultured Hippocampal mossy fiber 030109 nutrition & dietetics Neuronal Plasticity Post-tetanic potentiation Chemistry Depolarization General Medicine Rats Synaptic plasticity Mossy Fibers Hippocampal Synapses Female 030217 neurology & neurosurgery |
| Zdroj: | General physiology and biophysics. 36(3) |
| ISSN: | 0231-5882 |
| Popis: | The hippocampal mossy fibers contain a substantial quantity of loosely-bound zinc in their glutamatergic presynaptic vesicles, which is released in synaptic transmission processes. Despite the large number of studies about this issue, the zinc changes related to short and long-term forms of potentiation are not totally understood. This work focus on zinc signals associated with chemically-induced mossy fiber synaptic plasticity, in particular on postsynaptic zinc signals evoked by KCl depolarization. The signals were detected using the medium affinity fluorescent zinc indicator Newport Green. The application of large concentrations of KCl, 20 mM and 60 mM, in the extracellular medium evoked zinc potentiations that decreased and remained stable after washout of the first and the second media, respectively. These short and long-lasting enhancements are considered to be due to zinc entry into postsynaptic neurons. We have also observed that following established zinc potentiation, another application of 60 mM KCl only elicited further enhancement when combined with external zinc. These facts support the idea that the KCl-evoked presynaptic depolarization causes higher zinc release leading to zinc influx into the postsynaptic region. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|