Identification and characterization of four Drosophila suzukii cellularization genes and their promoters
| Autor: | Marc F. Schetelig, Syeda A. Jaffri, Jonas Schwirz, Carl Stein, Ying Yan |
|---|---|
| Přispěvatelé: | Publica |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine lcsh:QH426-470 Genetic Vectors Embryonic Development Genes Insect 01 natural sciences 03 medical and health sciences Morphogenesis Genetics Melanogaster Animals Lethal allele Promoter Regions Genetic Drosophila suzukii Gene Genetics (clinical) Spotted-wing Drosophila D. melanogaster S2 cells biology Schneider 2 cells Research fungi Tetracycline-off system Gene Expression Regulation Developmental Promoter biology.organism_classification lcsh:Genetics 010602 entomology 030104 developmental biology Transgenic embryonic sexing system Infertility DNA Transposable Elements Sterile insect technique Drosophila Genes Lethal Cellularization Blastoderm |
| Zdroj: | BMC Genetics BMC Genetics, Vol 21, Iss S2, Pp 1-10 (2020) |
| Popis: | BackgroundThe spotted-wing Drosophila (Drosophila suzukii) is a widespread invasive pest that causes severe economic damage to fruit crops. The early development ofD. suzukiiis similar to that of other Drosophilids, but the roles of individual genes must be confirmed experimentally. Cellularization genes coordinate the onset of cell division as soon as the invagination of membranes starts around the nuclei in the syncytial blastoderm. The promoters of these genes have been used in genetic pest-control systems to express transgenes that confer embryonic lethality. Such systems could be helpful in sterile insect technique applications to ensure that sterility (bi-sex embryonic lethality) or sexing (female-specific embryonic lethality) can be achieved during mass rearing. The activity of cellularization gene promoters during embryogenesis controls the timing and dose of the lethal gene product.ResultsHere, we report the isolation of theD. suzukiicellularization genesnullo, serendipity-α, bottleneckandslow-as-molassesfrom a laboratory strain. Conserved motifs were identified by comparing the encoded proteins with orthologs from other Drosophilids. Expression profiling confirmed that all four are zygotic genes that are strongly expressed at the early blastoderm stage. The 5′ flanking regions from these cellularization genes were isolated, incorporated intopiggyBacvectors and compared in vitro for the promoter activities. TheDsnullopromoter showed the highest activity in the cell culture assays usingD. melanogasterS2 cells.ConclusionsThe similarities in the gene coding and 5′ flanking sequence as well as in the expression pattern of the four cellularization genes betweenD. melanogasterandD. suzukii, suggest that conserved functions may be involved in both species. The high expression level at the early blastoderm stage of the four cellularization genes were confirmed, thus their promoters can be considered in embryonic lethality systems. While theDsnullopromoter could be a suitable candidate, all reported promoters here are subject to further in vivo analyses before constructing potential pest control systems. |
| Databáze: | OpenAIRE |
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