MALDI-TOF MS identification of Cimex lectularius and Cimex hemipterus bedbugs

Autor: Maureen Laroche, Philippe Parola, Jean Michel Berenger, Gladys Gazelle, Linda Benkacimi, Basma El Hamzaoui
Přispěvatelé: Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA), Institut de Recherche Biomédicale des Armées (IRBA)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)
Rok vydání: 2020
Předmět:
0301 basic medicine
Microbiology (medical)
Bedbugs
Cimex hemipterus
Databases
Factual
030106 microbiology
Zoology
Biology
Microbiology
03 medical and health sciences
[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system
Species level
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Genetics
medicine
Animals
[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
Molecular Biology
ComputingMilieux_MISCELLANEOUS
Ecology
Evolution
Behavior and Systematics
Arthropod identification
[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases
Ms analysis
medicine.disease
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
3. Good health
030104 developmental biology
Infectious Diseases
Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Identification (biology)
Cimex lectularius
Skin lesion
Zdroj: Infection, Genetics and Evolution
Infection, Genetics and Evolution, 2020, 85, pp.104536. ⟨10.1016/j.meegid.2020.104536⟩
Infection, Genetics and Evolution, Elsevier, 2020, 85, pp.104536. ⟨10.1016/j.meegid.2020.104536⟩
ISSN: 1567-1348
1567-7257
DOI: 10.1016/j.meegid.2020.104536
Popis: Bedbugs (Cimex lectularius and C. hemipterus) have reemerged as a major public health problem around the world. Their bites cause various skin lesions as well as discomfort and anxiety. Their role as potential vectors of various infectious agents is discussed. Accordingly, all suspected cases of bedbug infestations need to be documented thoroughly, with an unequivocal identification of the arthropods involved, if any are present. Although morphological identification is easily and quickly performed by entomologists or professionals, it can be challenging otherwise. Also, distinguishing Cimex lectularius and C. hemipterus requires entomological expertise. MALDI-TOF mass spectrometry has been recently presented as an additional tool for arthropod identification. In this study, we assess the use of MALDI-TOF MS for the identification of laboratory and wild strains of C. lectularius and C. hemipterus. Several body parts of laboratory reared C. lectularius specimens were used to develop a MALDI-TOF MS protocol for bedbug identification, which was later validated using five other laboratory and wild populations of C. hemipterus and C. lectularius. A total of 167C. lectularius and C. hemipterus bedbug specimens (98 laboratory specimens and 69 wild specimens) were submitted to MALDI-TOF MS analysis. 143/167 (85.63%) provided high quality MS spectra. The in-lab database was then upgraded with a total of 20 reference spectra from all bedbug populations and the rest of the MS spectra (123 bedbugs) were blind tested. All specimens were properly identified to the species level using MALDI-TOF MS and 86,25% (69/80) were aptly identified according to their origin with LSVs ranging from 1.867 to 2.861. MALDI-TOF MS appears as a reliable additional tool for the identification of these two anthropophilic species.
Databáze: OpenAIRE
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