Development of a Luminex assay for the detection of swine antibodies to non-structural proteins of foot-and-mouth disease virus

Autor: Chia-Ni Shih, Fan Lee, Yeou-Liang Lin, Ming-Chang Lee, Hsiang-Jung Tsai, Chu-Hsiang Pan, Tsu-Han Chen
Rok vydání: 2013
Předmět:
NSP
non-structural protein
Swine
Viral Nonstructural Proteins
Antibodies
Viral
PC
positive control
NC
negative control
SPF
specific-pathogen-free
Non-structural protein
PI
percentage of inhibition
Immunology and Allergy
T/C
test to control
Swine vesicular disease
dpi
days post infection
Specific-pathogen-free
Immunoassay
Swine Diseases
biology
Foot-and-mouth disease
Vaccination
HRP
horseradish peroxidase
ELISA
enzyme-linked immunosorbent assay
Microspheres
Swine Vesicular Disease Virus
Vesicular stomatitis virus
FMD
foot-and-mouth disease
Antibody
Foot-and-mouth disease virus
Vesicular Stomatitis
VSV
vesicular stomatitis virus
Immunology
Luminex assay
Sensitivity and Specificity
Article
Diagnosis
Differential
Swine Vesicular Disease
MFI
median fluorescent intensity
Enzyme-linked immunosorbent assay
medicine
Animals
xMAP
multi-analyte profiling
Viral Vaccines
SP
structural protein
medicine.disease
biology.organism_classification
equipment and supplies
Virology
respiratory tract diseases
OD
optical density
Antibody detection
Foot-and-Mouth Disease
biology.protein
FMDV
foot-and-mouth disease virus
SVDV
swine vesicular disease virus
Zdroj: Journal of Immunological Methods
ISSN: 1872-7905
Popis: Foot-and mouth disease (FMD), swine vesicular disease (SVD), and vesicular stomatitis (VS) are highly contagious vesicular diseases of swine but are not easy to differentiate clinically. For the purpose of instant detecting of FMD and differentiating it from the other vesicular diseases, a Luminex assay was developed. Sera from 64 infected, 307 vaccinated, and 280 naive pigs were tested by the Luminex assay. Diagnostic sensitivity of the assay was 100%. Diagnostic specificity of the assay was 98.7% in vaccinated pigs and 97.5% to 100% in naive pigs. Agreement between the results from the Luminex assay and those from a 3ABC polypeptide blocking ELISA was 96.3% with kappa statistics of 0.92. The Luminex assay can detect the immune response to NSP-3ABC in swine as early as eight days post-infection. Moreover, all of the 15 vaccinated but unprotected pigs were all detected by the Luminex assay. The results indicated that the Luminex assay has potential with specificity in detecting antibodies to FMDV 3ABC NSP and in distinguishing FMDV-infected pigs from with either SVDV or VSV.
Databáze: OpenAIRE
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