Deletion analysis of AGD1 reveals domains crucial for its plasma membrane recruitment and function in root hair polarity
| Autor: | Hiroo Fukuda, Jin Nakashima, Satoshi Naramoto, J. Alan Sparks, Elison B. Blancaflor, Bibi Rafeiza Khan, Cheol Min Yoo |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cytoplasm Recombinant Fusion Proteins Green Fluorescent Proteins Root hair Plant Roots 01 natural sciences Green fluorescent protein Structure-Activity Relationship 03 medical and health sciences Phosphatidylinositol Phosphates Protein Domains Botany Arabidopsis thaliana Tip growth Sequence Deletion Polarity (international relations) biology Arabidopsis Proteins Cell Membrane GTPase-Activating Proteins Cell Biology biology.organism_classification Cell biology Pleckstrin homology domain 030104 developmental biology Mutation Function (biology) 010606 plant biology & botany |
| Zdroj: | Journal of Cell Science. |
| ISSN: | 1477-9137 0021-9533 |
| Popis: | AGD1, a plant ACAP-type ADP-ribosylation factor-GTPase activating protein (ARF-GAP), functions in specifying root hair polarity in Arabidopsis thaliana. To better understand how AGD1 modulates root hair growth, we generated full length and domain-deleted AGD1-green fluorescent protein (GFP) constructs, and followed their localization during root hair development. AGD1-GFP localized to the cytoplasm and was recruited to specific regions of the root hair plasma membrane (PM). Distinct PM AGD1-GFP signal was first detected along the site of root hair bulge formation. The construct continued to mark the PM at the root hair apical dome but only during periods of reduced growth. During rapid tip-growth, AGD1-GFP labeled the PM of the lateral flanks and dissipated from the apical-most PM. Deletion analysis and a single domain GFP fusion revealed that the pleckstrin homology (PH) domain is the minimal unit required for recruitment of AGD1 to the PM. Our results indicate that differential recruitment of AGD1 to specific PM domains is an essential component of the membrane trafficking machinery that facilitates root hair developmental phase transitions and responses to changes in the root microenvironment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|