Visualization of Three Sclerotiniaceae Species Pathogenic on Onion Reveals Distinct Biology and Infection Strategies
| Autor: | Maikel B F Steentjes, Sander Langebeeke, Olga E. Scholten, Jan A. L. van Kan, Sebastian Tonn, Hilde Coolman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Allium cepa Botrytis aclada Botrytis squamosa white rot Plant Roots 01 natural sciences fluorescence microscopy Conidium lcsh:Chemistry Infection biology Sclerotium cepivorum Onions lcsh:QH301-705.5 onion Spectroscopy Fluorescence microscopy biology food and beverages General Medicine Computer Science Applications Horticulture neck rot Neck rot Botrytis Onion Sclerotium Leaf blight Catalysis Article Inorganic Chemistry 03 medical and health sciences Ascomycota Sclerotiniaceae Blight Physical and Theoretical Chemistry Molecular Biology Laboratorium voor Nematologie Plant Diseases Organic Chemistry fungi biology.organism_classification Laboratorium voor Phytopathologie Plant Breeding 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Laboratory of Phytopathology infection biology White rot EPS Laboratory of Nematology leaf blight 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 4 International Journal of Molecular Sciences, Vol 22, Iss 1865, p 1865 (2021) International Journal of Molecular Sciences, 22(4) International Journal of Molecular Sciences 22 (2021) 4 |
| ISSN: | 1422-0067 1661-6596 |
| Popis: | Botrytis squamosa, Botrytis aclada, and Sclerotium cepivorum are three fungal species of the family Sclerotiniaceae that are pathogenic on onion. Despite their close relatedness, these fungi cause very distinct diseases, respectively called leaf blight, neck rot, and white rot, which pose serious threats to onion cultivation. The infection biology of neck rot and white rot in particular is poorly understood. In this study, we used GFP-expressing transformants of all three fungi to visualize the early phases of infection. B. squamosa entered onion leaves by growing either through stomata or into anticlinal walls of onion epidermal cells. B. aclada, known to cause post-harvest rot and spoilage of onion bulbs, did not penetrate the leaf surface but instead formed superficial colonies which produced new conidia. S. cepivorum entered onion roots via infection cushions and appressorium-like structures. In the non-host tomato, S. cepivorum also produced appressorium-like structures and infection cushions, but upon prolonged contact with the non-host the infection structures died. With this study, we have gained understanding in the infection biology and strategy of each of these onion pathogens. Moreover, by comparing the infection mechanisms we were able to increase insight into how these closely related fungi can cause such different diseases. |
| Databáze: | OpenAIRE |
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