Protein Labelling with Versatile Phosphorescent Metal Complexes for Live Cell Luminescence Imaging
| Autor: | Janine L. James, Anthony R. White, Timothy U. Connell, Paul S. Donnelly |
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| Rok vydání: | 2015 |
| Předmět: |
Luminescence
chemistry.chemical_element Ligands Catalysis chemistry.chemical_compound Coordination Complexes Cell Line Tumor Organic chemistry Humans Bifunctional chemistry.chemical_classification Cycloaddition Reaction Staining and Labeling Biomolecule Organic Chemistry Proteins General Chemistry Photobleaching Combinatorial chemistry Cycloaddition Ruthenium chemistry Luminescent Measurements Click chemistry Click Chemistry Phosphorescence |
| Zdroj: | Chemistry (Weinheim an der Bergstrasse, Germany). 21(40) |
| ISSN: | 1521-3765 |
| Popis: | To take advantage of the luminescent properties of d(6) transition metal complexes to label proteins, versatile bifunctional ligands were prepared. Ligands that contain a 1,2,3-triazole heterocycle were synthesised using Cu(I) catalysed azide-alkyne cycloaddition "click" chemistry and were used to form phosphorescent Ir(III) and Ru(II) complexes. Their emission properties were readily tuned, by changing either the metal ion or the co-ligands. The complexes were tethered to the metalloprotein transferrin using several conjugation strategies. The Ir(III)/Ru(II)-protein conjugates could be visualised in cancer cells using live cell imaging for extended periods without significant photobleaching. These versatile phosphorescent protein-labelling agents could be widely applied to other proteins and biomolecules and are useful alternatives to conventional organic fluorophores for several applications. |
| Databáze: | OpenAIRE |
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