Two different forms of p53 localized differently within cells of urogenital tumours
| Autor: | Helga Selter, Mathias Montenarh, Thomas Zwergel, Zacharia Teleb, Gerhard Unteregger, Jens Benninghoff, Bernd Wullich, Sabine Kartarius |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Cancer Research Immunoprecipitation Nucleolus medicine.drug_class Immunocytochemistry Blotting Western Biology Immunofluorescence Monoclonal antibody Mutant protein medicine Tumor Cells Cultured Humans Cell Nucleus Microscopy Confocal medicine.diagnostic_test Prostatic Neoplasms Subcellular localization Molecular biology Immunohistochemistry Cell nucleus medicine.anatomical_structure Oncology Urinary Bladder Neoplasms Karyotyping Mutation Cancer research Tumor Suppressor Protein p53 Cell Nucleolus |
| Zdroj: | Cancer letters. 144(1) |
| ISSN: | 0304-3835 |
| Popis: | We analyzed the subcellular localization of p53 in prostate and bladder carcinoma cells. Using laser scanning microscopy and PAb1620, a monoclonal antibody recognizing the wildtype conformation of p53, and another monoclonal antibody directed against the mutant conformation of the protein (PAb240), we found two different subsets of p53 within the same cell. The wildtype subgroup was found in the nucleolus, whereas the mutant protein was confined to the nucleus. The results obtained by immunofluorescence were verified by Western blot analysis and immunoprecipitation. Thus, our findings demonstrate an unusual subcellular localization pattern of p53 in prostate and bladder cancer cells which may indicate another mechanism of inactivation of p53. |
| Databáze: | OpenAIRE |
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