Basicity of the amino groups of the aminoglycoside amikacin using capillary electrophoresis and coupled CE-MS-MS techniques
| Autor: | H Gao, Pamela K. Jensen, J C McDonald, P T Glink, Liljiana Pasa-Tolic, Ravi S. Kane, Richard D. Smith, George M. Whitesides, R G Chapman |
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| Rok vydání: | 2001 |
| Předmět: |
Molecular Sequence Data
Analytical chemistry Acid dissociation constant Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Capillary electrophoresis Spectroscopy Fourier Transform Infrared medicine Carbohydrate Conformation Molecule Amines Amikacin Antibacterial agent Chromatography Aminoglycoside Electrophoresis Capillary Acetylation Hydrogen-Ion Concentration Anti-Bacterial Agents Electrophoresis chemistry Carbohydrate Sequence Functional group medicine.drug |
| Zdroj: | Analytical chemistry. 73(16) |
| ISSN: | 0003-2700 |
| Popis: | This paper describes the use of capillary electrophoresis (CE), and coupled CE and mass spectrometric techniques, to measure the values of the pKa of the amino groups of the aminoglycoside antibiotic amikacin and of its acetylated derivatives. These values of pKa (8.4, 6.7, 9.7, 8.4) were determined by measuring the electrophoretic mobilities of the molecules as a function of pH; they are within 0.7 unit of certain values reported in the literature (by 13C and 15N NMR spectroscopies) but resolved ambiguities left by these earlier studies. The range of values of pKa of amino groups also indicates the complex dependence of the acidity of a functional group (and thus the extent of ionization at a specified value of pH) on the molecular environment of that group. |
| Databáze: | OpenAIRE |
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