The Routine Determination of the Endogenous Thrombin Potential, First Results in Different Forms of Hyper- and Hypocoagulability
| Autor: | Dirk T. S. Rijkers, R. W. C. Knebel, H. C. Hemker, J. Michiels, Simone J.H. Wielders, V. Kakkar, Suzette Beguin, J.-P. Cambus, M. Mukherjee |
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| Přispěvatelé: | Biochemie |
| Rok vydání: | 1997 |
| Předmět: | |
| Zdroj: | Thrombosis and Haemostasis, 77(4), 629-636. Georg Thieme Verlag Scopus-Elsevier |
| ISSN: | 2567-689X 0340-6245 |
| DOI: | 10.1055/s-0038-1656024 |
| Popis: | SummaryThe area under the thrombin generation curve (the endogenous thrombin potential; ETP) has been proposed as a parameter for plasma-based hypercoagulability and to monitor anticoagulant treatment. We present an ETP assay for the routine laboratory using a centrifugal analyser. Throughput is 30 samples/h, within and between run imprecision is 4-5.6%. Suitable substrates were developed for the ranges of 10-500% and 2-100% of normal.Independent of tissue factor concentration (if >4 pM), the normal value of the extrinsic ETP is 384.8 ±51.7 nM.min. The intrinsic ETP, triggered by ellagic acid, is 414 ± 41 nM.min.The ETP is decreased to 15 and 35% of normal by oral anticoagulation (INR 2.5-4.0) and by heparin administration (APTT 1.5-2.5 X control).The ETP is increased in untreated subjects with congenital antithrombin deficiency and in women using oral contraceptives. In deep vein thrombosis (phlebographically confirmed), it is increased by 29.4% (extrinsic) and 53% (intrinsic). In (angiographically assessed) coronary artery disease the increase is by 10% and 17% respectively. |
| Databáze: | OpenAIRE |
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