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Saccharomyces diastaticus cells were immobilized onto beech wood chips of different particle size and three pH values. pH values in the range 5.0-6.0, and 1.84-1.92 mm particle size had a positive effect on the immobilization process. The chosen carrier--1.84 mm-sized wood chips adsorbed 150 mg dry cell mass per g dry carrier mass. The Gibbs free energy and the activation energy for the first (monolayer) and second (multilayer) immobilization stages was 4581, 19,090 and 8590 J g mol-1, respectively. The kinetics of immobilized cell systems in ethanol production have been studied in a packed bed-reactor. Ethanol production and the respiration quotient (RQ) were at a maximum at a dilution rate of 0.16/h. The reactor was operated under steady-state conditions for 30 d at the dilution rate 0.16/h. |
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