Immune Ontogeny and Engraftment Receptivity in the Sheep Fetus

Autor: Nicole M. Frederick, David Thain, Alireza Torabi, Jessica L. Skopal-Chase, Esmail D. Zanjani, Mihai Cenariu, Daria Groza, John S. Pixley, Anupama Bhat
Rok vydání: 2009
Předmět:
CD4-Positive T-Lymphocytes
Male
Embryology
Time Factors
Ontogeny
medicine.medical_treatment
Transplantation
Heterologous
Immunology
Receptivity
Gestational Age
Thymus Gland
Hematopoietic stem cell transplantation
CD8-Positive T-Lymphocytes
Biology
Biochemistry
Immune tolerance
Fetal Development
Andrology
Fetus
Immune system
medicine
Animals
Humans
Radiology
Nuclear Medicine and imaging
Original Paper
Pregnancy
Sheep
Hematopoietic Stem Cell Transplantation
Hematopoietic stem cell
Obstetrics and Gynecology
Cell Biology
Hematology
General Medicine
biochemical phenomena
metabolism
and nutrition
medicine.disease
Transplantation
medicine.anatomical_structure
Graft-versus-host disease
Lymphatic system
surgical procedures
operative
Pediatrics
Perinatology and Child Health
biology.protein
Gestation
Female
Antibody
Zdroj: ResearcherID
ISSN: 1421-9964
1015-3837
DOI: 10.1159/000203399
Popis: The therapeutic application of in utero hematopoietic stem cell (HSC) transplantation (IUHSCT) is theoretically attractive for definitive treatment of congenital disease states. Investigating this technique in sheep, we have previously shown long-term engraftment and expression of both allogeneic and xenogeneic donor cells without cytoablation and, under appropriate conditions, without GVHD. The theoretical basis for IUHSCT is the well-recognized immune receptivity of the fetus to engraftment of donor cells. Engraftment and long-term expression of donor human and allogeneic sheep HSC reliably occur in the fetal sheep model if the IUHSCT is performed prior to day 71 of gestation (term: 145 days), during the period of presumed immuno-naïveté. Investigations using alternate animals have also noted that gestational age at transplantation is critical to achieving long-term engraftment, presumably as a result of inducing durable immune tolerance to the donor. Despite this presumption, however, the biologic explanation for fetal receptivity to donor engraftment and subsequent long-term tolerance following transplantation early in gestation is not known. In the present studies, we investigated the role fetal immune ontogeny plays in the induction of tolerance following IUHSCT in sheep. To this end, we performed parallel experiments examining engraftment receptivity of fetal sheep to allogeneic and xenogeneic HSC and the appearance of immune phenotypes in fetal sheep lymphoid organs at varying gestational ages (days 39 to birth), attempting to draw correlations between the appearance/absence of specific immune cells and the ability to achieve durable engraftment and immune tolerance. Engraftment receptivity was determined 60 days post-transplantation at different time points in sheep fetal gestation, while immune phenotypes were determined by flow cytometry using commercially available antibodies to immune cell surface markers. Our results indicate that the fetus is largely non-receptive to engraftment of both allogeneic and xenogeneic donor HSC prior to day 52 gestation and possesses a peak in engraftment receptivity between days 64–71 of gestation, which rapidly declines thereafter. With respect to the developing fetal immune system, the period of peak engraftment receptivity was associated with the expression of CD45 on all cells in the thymus. Double-positive and single-positive CD4 and CD8 cells began appearing in the thymus just prior (day 45 of gestation) to the beginning of the engraftment window, while single-positive CD4 or CD8 cells did not begin appearing in peripheral organs until late in the engraftment period, suggesting deletional mechanisms predominate during this time. In a similar fashion, surface IgM (sIgM)+ cells in the thymus were the first to express CD45, commencing expression around day 45 of gestation, with a comparable delay in the appearance of IgM+/CD45+ cells in the peripheral blood and spleen until late in the engraftment window. These findings support a central role for the thymus in multilineage immune cell maturation during the period of fetal transplantation receptivity. Further, they suggest that fetal engraftment receptivity/long-term engraftment and expression following IUHSCT is due to gestational age-dependent deletional tolerance. Further, our findings suggest that IUHSCT in humans may be more successful if performed during the comparable period in human gestation.
Databáze: OpenAIRE
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