Biological and molecular properties of a pathotype P-1 and a pathotype P-4 isolate of pea seed-borne mosaic virus
| Autor: | W. G. Dougherty, K. E. Keller, R. O. Hampton, I. E. Johansen |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Potyvirus Genes Recessive Genome Viral Genes Plant Polymerase Chain Reaction Chenopodium quinoa Pisum Open Reading Frames Viral Proteins Sativum Virology Cloning Molecular Plant Diseases Infectivity biology Peas Nucleic acid sequence Chromosome Mapping food and beverages biology.organism_classification Seeds Pea seed-borne mosaic virus Myzus persicae |
| Zdroj: | Johansen, I E, Keller, K E, Dougherty, W G & Hampton, R O 1996, ' Biological and molecular properties of a pathotype P-1 and a pathotype P-4 isolate of pea seed-borne mosaic virus ' Journal of General Virology, vol. 77, pp. 1329-1333 . |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-77-6-1329 |
| Popis: | Two isolates of pea seed-borne mosaic potyvirus, DPD1 and NY, were identified as pathotypes P-1 and P-4, respectively, by their infectivity on Pisum sativum L. lines homozygous for the recessive resistance genes sbm-1 and sbm-4. The two isolates differed in several biological characteristics. DPD1 induced transient vein clearing, downward rolling of leaflets and internode shortening on P. sativum, whereas NY only caused a slight growth reduction. DPD1 moved systemically in Chenopodium quinoa whereas NY was restricted to inoculated leaves. DPD1 was frequently transmitted by seeds whereas NY was rarely seed-transmitted: 24% and 0.3%, respectively, in P. sativum '549'. Both DPD1 and NY were transmitted by aphids (Myzus persicae), though a DAG triplet was not present in the N terminus of the coat protein. The nucleotide sequence and deduced amino acid sequence of NY were determined and compared to the corresponding sequences of DPD1. |
| Databáze: | OpenAIRE |
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