Pressure induced nucleus DNA fragmentation
| Autor: | Barbara Gajda, Piotr Pietrewicz, Jacek A. Modlinski, Piotr Sieczyński, Pawel Greda, Grzegorz Mrugacz, Cezary Grygoruk, Izabela Grad |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Apoptosis DNA Fragmentation Biology Mice Technical Innovations Pregnancy In Situ Nick-End Labeling Pressure Genetics medicine Animals Blastocyst reproductive and urinary physiology Genetics (clinical) Cell Nucleus Obstetrics and Gynecology General Medicine Molecular biology Human genetics Nuclear DNA Mice Inbred C57BL Cell nucleus medicine.anatomical_structure Reproductive Medicine embryonic structures DNA fragmentation Female Nucleus Developmental Biology |
| Zdroj: | Journal of Assisted Reproduction and Genetics. 28:363-368 |
| ISSN: | 1573-7330 1058-0468 |
| DOI: | 10.1007/s10815-010-9525-1 |
| Popis: | The present study was designed to investigate the impact of pressure on nuclear DNA integrity in viable cells of mouse blastocysts.The blastocysts of hybrid F1 females [(C57Bl/10 J × CBA-H);N = 15] aged 2-3 months were exposed into the pressure impulse lasting ~0.021 s and characterized by a positive pressure peak of ~76 mmHg. The nuclear DNA fragmentation index of mouse blastocysts was assessed by TUNEL assay within 60 s after exposure to pressure impulse.The mean nuclear DNA fragmentation index was significantly higher in the experimental group (83%) than in the control group (19.7%); p 0.001.A low magnitude pressure impulse can induce nuclear DNA fragmentation in mouse blastocysts. The compression and decompression forces appearing during pressure fluctuations are responsible for the observed DNA shearing. |
| Databáze: | OpenAIRE |
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