Nonreplicating Vaccines Can Protect African Green Monkeys From the Memphis 37 Strain of Respiratory Syncytial Virus
| Autor: | Julia Li, J. Erik Johnson, Derek John Falconer, Kate West, Kathrin U. Jansen, Oscar Maldonado, Susanne Lang, Jim E. Eyles, Cheryl S. Kotash, Shawn R. Makinen, Phil White, Risini D. Weeratna, James R. Merson, Thomas P. Brown, Paul Wright, George J. Smith, Clare Lees, Susan E. Witko, Paul Cockle, Vidia Roopchand, Peter T. Loudon, Shakuntala Megati, Maninder K. Sidhu |
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| Rok vydání: | 2013 |
| Předmět: |
T-Lymphocytes
medicine.medical_treatment Respiratory Syncytial Virus Infections Antibodies Viral Virus Replication Bronchoalveolar Lavage Virus Viral vector law.invention Random Allocation Viral Proteins Adjuvants Immunologic law Chlorocebus aethiops Respiratory Syncytial Virus Vaccines medicine Animals Immunology and Allergy Lung biology Adenoviruses Human Vesiculovirus Viral Load Virology Respiratory Syncytial Viruses Vaccination Infectious Diseases Immunization biology.protein Recombinant DNA Antibody Viral Fusion Proteins Adjuvant Viral load Granulocytes |
| Zdroj: | Journal of Infectious Diseases. 208:319-329 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/jit169 |
| Popis: | Background. We evaluated the immunological responses of African green monkeys immunized with multiple F and G protein–based vaccines and assessed protection against the Memphis 37 strain of respiratory syncytial virus (RSV). Methods. Monkeys were immunized with F and G proteins adjuvanted with immunostimulatory (CpG) oligodeoxyribonucleotides admixed with either Alhydrogel or ISCOMATRIX adjuvant. Delivery of F and G proteins via replication incompetent recombinant vesicular stomatitis viruses (VSVs) and human adenoviruses was also evaluated. Mucosally or parenterally administered recombinant adenoviruses were used in prime-boost regimens with adjuvanted proteins or recombinant DNA. Results. Animals primed by intranasal delivery of recombinant adenoviruses, and boosted by intramuscular injection of adjuvanted F and G proteins, developed neutralizing antibodies and F/G protein–specific T cells and were protected from RSV infection. Intramuscular injections of Alhydrogel (plus CpG) adjuvanted F and G proteins reduced peak viral loads in the lungs of challenged monkeys. Granulocyte numbers were not significantly elevated, relative to controls, in postchallenge bronchoalveolar lavage samples from vaccinated animals. Conclusions. This study has validated the use of RSV (Memphis 37) in an African green monkey model of intranasal infection and identified nonreplicating vaccines capable of eliciting protection in this higher species challenge model. |
| Databáze: | OpenAIRE |
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