Over-expression of CDH22 is associated with tumor progression in colorectal cancer
| Autor: | Weizhe Gao, Jianming Li, Yan-qing Ding, Jun Zhou, Yu-Hong Liu, Juanzhi Chen |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Oncology
Male medicine.medical_specialty Colorectal cancer Blotting Western Morphogenesis Mice Nude Mouse model of colorectal and intestinal cancer Metastasis Mice In vivo Cell Movement Internal medicine Cell Line Tumor medicine Biomarkers Tumor Animals Humans Neoplasm Invasiveness RNA Messenger Cell Proliferation Gene knockdown Cadherin business.industry Reverse Transcriptase Polymerase Chain Reaction General Medicine Middle Aged medicine.disease Cadherins Flow Cytometry Immunohistochemistry Xenograft Model Antitumor Assays Up-Regulation Tumor progression Disease Progression Female RNA Interference business Colorectal Neoplasms |
| Zdroj: | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 30(3) |
| ISSN: | 1423-0380 |
| Popis: | CDH22, a member of the cadherins family, is highly expressed in the pituitary gland and the brain. It has been previously found that CDH22 is involved in morphogenesis and tissue formation in neural and non-neural cells of the brain and neuroendocrine organ. However, little is known about its role in colorectal cancer. Here the role of CDH22 in colorectal tumor was tested, and effects of inhibition of CDH22 expression on proliferation and metastasis were examined. We found that CDH22 was over-expressed at both transcriptional and translational levels in colorectal cancer and lymphatic metastasis of colorectal carcinoma, compared with normal colorectal mucosa. CDH22 over-expression was significantly associated with invasion and metastasis of colorectal cancer both at protein and mRNA levels. CDH22 knockdown partially blocked proliferation of colorectal cancer cells in vivo and in vitro. CDH22 knockdown was sufficient to attenuate invasion of colorectal cancer cells and inhibit tumor metastasis in a mouse model of colon surgical orthotopic implantation. Our results reveal for the first time a new role of CDH22 in progression of colorectal cancer. |
| Databáze: | OpenAIRE |
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