Simultaneous immobilization of glucose oxidase on the surface and cavity of hollow gold nanospheres as labels for highly sensitive electrochemical immunoassay of tumor marker
| Autor: | Ruo Yuan, Huilan Su, Wen Jiang, Xiaoqi Ran, Yaqin Chai, Zhongju Song, Xin Che |
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| Rok vydání: | 2011 |
| Předmět: |
Analyte
Conductometry Surface Properties Biomedical Engineering Biophysics Analytical chemistry Biosensing Techniques Micelle Glucose Oxidase Biomarkers Tumor Electrochemistry medicine Humans Glucose oxidase Immunoassay Detection limit Chromatography Staining and Labeling medicine.diagnostic_test biology Chemistry Equipment Design General Medicine Enzymes Immobilized Primary and secondary antibodies humanities Amperometry Carcinoembryonic Antigen Equipment Failure Analysis Linear range biology.protein Gold Nanospheres Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 26:2776-2780 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2010.10.039 |
| Popis: | A novel tracer, glucose oxidase (GOD)-functionalized hollow gold nanospheres encapsulating glucose oxidase (Au shell @GOD), was designed to label the ferrocenemonocarboxylic-grafted secondary antibodies (Fc@Ab 2 ) for highly sensitive detection of tumor marker using carboxyl group functionalized multiwall carbon nanotubes as platform. Initially, Au shell @GOD was synthesized specially by reverse micelle approach, and then the labeling of antibody and the preparation of GOD-functionalized Au shell @GOD were performed by one-pot assembly of Fc@Ab 2 and GOD on the surface of Au shell @GOD. The ferrocene used to label antibodies acted as a mediator of electron transfer between GOD and electrode surface. The high-content glucose oxidase in the tracer (on the surface and in the cavity) could significantly amplify the amperometric signal for sandwich-type immunoassay. Using carcinoembryonic antigen (CEA) as model analyte, the designed tracer showed linear range from 0.02 to 5.0 ng mL −1 with the detection limit down to 6.7 pg mL −1 . The assay results of serum samples with the proposed method were in an acceptable agreement with the reference values. The new protocol showed acceptable stability and reproducibility, high sensitivity, and good precision, which could provide a promising potential for clinical screening and diagnosis of tumor disease. |
| Databáze: | OpenAIRE |
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