Quantitative proteomic analysis of the sugarcane defense responses incited by Acidovorax avenae subsp. avenae causing red stripe

Autor: Philippe C. Rott, Ahmad Ali, Jing-Ru Zhou, San-Ji Gao, Hui-Dong Sun, Talha Javed, Hua-Ying Fu
Přispěvatelé: Fujian Agriculture and Forestry University (FAFU), University of South Florida [Tampa] (USF), Plant Health Institute of Montpellier (UMR PHIM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), earmarked fund for the China Agricultural Research System (grant number CARS-170302), Special Fund for Science and Technology Innovation in Fujian Agriculture and Forestry University (FAFU) China (grant number CXZX2016170), Director's Innovation Fund in National Engineering Research Center for Sugarcane, FAFU, China (grant number KJG16005M) .
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0106 biological sciences
Gene isoform
avenae
Proteomics
Protein subunit
01 natural sciences
chemistry.chemical_compound
Maladie des plantes
Analyse quantitative
Biosynthesis
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
protéomique
KEGG
mécanisme de défense
Transcription factor
Epoxide Hydrolase 1
H20 - Maladies des plantes
Acidovorax avenae subsp
2. Zero hunger
biology
Differentially expressed proteins
010405 organic chemistry
Acidovorax
Réponse de la plante
food and beverages
Résistance aux maladies
biology.organism_classification
Saccharum
0104 chemical sciences
[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
chemistry
Biochemistry
iTRAQ
Red stripe
Canne à sucre
Agronomy and Crop Science
010606 plant biology & botany
Zdroj: Industrial Crops and Products
Industrial Crops and Products, Elsevier, 2021, 162, pp.113275. ⟨10.1016/j.indcrop.2021.113275⟩
ISSN: 0926-6690
Popis: International audience; The causal agent of red stripe, Acidovorax avenae subsp. avenae (Aaa), can incite significant economic losses during sugarcane production. Proteomics is a useful tool for the comprehensive identification of defense response proteins in plants. In this study, isobaric tags for relative and absolute quantification (iTRAQ)-based proteomics was used to identify proteins that were produced in sugarcane in defense to Aaa in two Saccharum spp. cultivars: ROC22 (resistant to red stripe) and MT11-610 (susceptible). Defense response was evaluated at 0 h (mock inoculation) and 24 h after inoculation with the bacterial pathogen, and 10,502 high-confidence proteins and 1962 differentially expressed proteins (DEPs) were identified. These DEPs included 1027 proteins (671 upregulated, 354 downregulated) in ROC22 and 1130 proteins (566 upregulated, 564 downregulated) in MT11-610. Gene ontology analysis revealed that most DEPs were clustered in the metabolic, single-organism metabolic, and oxidation-reduction processes, and in catalytic activity. In a KEGG analysis, these proteins belonged to common pathways such as secondary metabolite biosynthesis and biosynthesis of phenylpropanoids. Thirty-six DEPs were identified, especially epoxide hydrolase 1, dynamin-related protein 1C-like, cytochrome oxidase subunit I, FK506-binding protein, acetoacetyl CoA thiolase isoform 2, anthocyanidin 3-o-glucosyltransferase, ubiquitin carboxyl-terminal hydrolase 4, protein disulfide isomerase-like protein, and jumonji-like transcription factor family protein. These nine proteins were characterized by enhanced expression with >= 1.5-fold increases in resistant ROC22 versus susceptible MT11-610, and are therefore biomarker candidates in the sugarcane response against Aaa. These data could be very useful in molecular breeding for sugarcane resistance to red stripe.
Databáze: OpenAIRE
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