Quantitative proteomic analysis of the sugarcane defense responses incited by Acidovorax avenae subsp. avenae causing red stripe
Autor: | Philippe C. Rott, Ahmad Ali, Jing-Ru Zhou, San-Ji Gao, Hui-Dong Sun, Talha Javed, Hua-Ying Fu |
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Přispěvatelé: | Fujian Agriculture and Forestry University (FAFU), University of South Florida [Tampa] (USF), Plant Health Institute of Montpellier (UMR PHIM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), earmarked fund for the China Agricultural Research System (grant number CARS-170302), Special Fund for Science and Technology Innovation in Fujian Agriculture and Forestry University (FAFU) China (grant number CXZX2016170), Director's Innovation Fund in National Engineering Research Center for Sugarcane, FAFU, China (grant number KJG16005M) . |
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
Gene isoform avenae Proteomics Protein subunit 01 natural sciences chemistry.chemical_compound Maladie des plantes Analyse quantitative Biosynthesis [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] protéomique KEGG mécanisme de défense Transcription factor Epoxide Hydrolase 1 H20 - Maladies des plantes Acidovorax avenae subsp 2. Zero hunger biology Differentially expressed proteins 010405 organic chemistry Acidovorax Réponse de la plante food and beverages Résistance aux maladies biology.organism_classification Saccharum 0104 chemical sciences [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy chemistry Biochemistry iTRAQ Red stripe Canne à sucre Agronomy and Crop Science 010606 plant biology & botany |
Zdroj: | Industrial Crops and Products Industrial Crops and Products, Elsevier, 2021, 162, pp.113275. ⟨10.1016/j.indcrop.2021.113275⟩ |
ISSN: | 0926-6690 |
Popis: | International audience; The causal agent of red stripe, Acidovorax avenae subsp. avenae (Aaa), can incite significant economic losses during sugarcane production. Proteomics is a useful tool for the comprehensive identification of defense response proteins in plants. In this study, isobaric tags for relative and absolute quantification (iTRAQ)-based proteomics was used to identify proteins that were produced in sugarcane in defense to Aaa in two Saccharum spp. cultivars: ROC22 (resistant to red stripe) and MT11-610 (susceptible). Defense response was evaluated at 0 h (mock inoculation) and 24 h after inoculation with the bacterial pathogen, and 10,502 high-confidence proteins and 1962 differentially expressed proteins (DEPs) were identified. These DEPs included 1027 proteins (671 upregulated, 354 downregulated) in ROC22 and 1130 proteins (566 upregulated, 564 downregulated) in MT11-610. Gene ontology analysis revealed that most DEPs were clustered in the metabolic, single-organism metabolic, and oxidation-reduction processes, and in catalytic activity. In a KEGG analysis, these proteins belonged to common pathways such as secondary metabolite biosynthesis and biosynthesis of phenylpropanoids. Thirty-six DEPs were identified, especially epoxide hydrolase 1, dynamin-related protein 1C-like, cytochrome oxidase subunit I, FK506-binding protein, acetoacetyl CoA thiolase isoform 2, anthocyanidin 3-o-glucosyltransferase, ubiquitin carboxyl-terminal hydrolase 4, protein disulfide isomerase-like protein, and jumonji-like transcription factor family protein. These nine proteins were characterized by enhanced expression with >= 1.5-fold increases in resistant ROC22 versus susceptible MT11-610, and are therefore biomarker candidates in the sugarcane response against Aaa. These data could be very useful in molecular breeding for sugarcane resistance to red stripe. |
Databáze: | OpenAIRE |
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