A serum factor induces insulin-independent translocation of GLUT4 to the cell surface which is maintained in insulin resistance
| Autor: | Marion Berenguer, Sophie Giorgetti-Peraldi, Roland Govers, Laurène Martinez, Yannick Le Marchand-Brustel |
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| Přispěvatelé: | Centre méditerranéen de médecine moléculaire (C3M), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA), Université de Poitiers - Faculté de Médecine et de Pharmacie, Université de Poitiers, Centre méditérannéen de médecine moléculaire (C3M), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Signalisation et pathologies (IFR50), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre Hospitalier Universitaire de Nice (CHU Nice)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Nutrition, obésité et risque thrombotique (NORT), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre Hospitalier Universitaire de Nice (CHU Nice)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Universitaire de Nice (CHU Nice)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
MESH: Hepatocyte Growth Factor
medicine.medical_treatment Glucose uptake MESH: Insulin-Like Growth Factor I lcsh:Medicine [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Biochemistry Transmembrane Transport Proteins Myoblasts Mice Endocrinology 0302 clinical medicine Insulin receptor substrate Molecular Cell Biology Adipocytes Signaling in Cellular Processes Insulin MESH: Animals Insulin-Like Growth Factor I lcsh:Science Platelet-Derived Growth Factor 0303 health sciences Glucose Transporter Type 4 Multidisciplinary Hepatocyte Growth Factor MESH: Platelet-Derived Growth Factor musculoskeletal system MESH: Gene Expression Regulation [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] Insulin oscillation Insulin signaling MESH: Glucose Protein Transport MESH: Insulin Resistance Blood Chemistry Medicine Membranes and Sorting hormones hormone substitutes and hormone antagonists Research Article Signal Transduction medicine.medical_specialty MESH: Protein Transport MESH: Insulin Biology 03 medical and health sciences Insulin resistance 3T3-L1 Cells Internal medicine Receptors Transferrin medicine Animals Humans MESH: Receptors Transferrin [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] MESH: Mice Glucose signaling MESH: Adipocytes 030304 developmental biology Diabetic Endocrinology Plasma Proteins MESH: Humans lcsh:R Glucose transporter Proteins Biological Transport [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Diabetes Mellitus Type 2 medicine.disease Hormones MESH: 3T3-L1 Cells Transmembrane Proteins Cell membranes Insulin receptor Metabolism Glucose Gene Expression Regulation biology.protein lcsh:Q MESH: Glucose Transporter Type 4 030217 neurology & neurosurgery GLUT4 |
| Zdroj: | PLoS ONE PLoS ONE, 2010, 5 (12), pp.e15560. ⟨10.1371/journal.pone.0015560⟩ PLoS ONE, Vol 5, Iss 12, p e15560 (2010) PLoS ONE, Public Library of Science, 2010, 5 (12), pp.e15560. ⟨10.1371/journal.pone.0015560⟩ |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0015560⟩ |
| Popis: | International audience; In response to insulin, glucose transporter GLUT4 translocates from intracellular compartments towards the plasma membrane where it enhances cellular glucose uptake. Here, we show that sera from various species contain a factor that dose-dependently induces GLUT4 translocation and glucose uptake in 3T3-L1 adipocytes, human adipocytes, myoblasts and myotubes. Notably, the effect of this factor on GLUT4 is fully maintained in insulin-resistant cells. Our studies demonstrate that the serum-induced increase in cell surface GLUT4 levels is not due to inhibition of its internalization and is not mediated by insulin, PDGF, IGF-1, or HGF. Similarly to insulin, serum also augments cell surface levels of GLUT1 and TfR. Remarkably, the acute effect of serum on GLUT4 is largely additive to that of insulin, while it also sensitizes the cells to insulin. In accordance with these findings, serum does not appear to activate the same repertoire of downstream signaling molecules that are implicated in insulin-induced GLUT4 translocation. We conclude that in addition to insulin, at least one other biological proteinaceous factor exists that contributes to GLUT4 regulation and still functions in insulin resistance. The challenge now is to identify this factor. |
| Databáze: | OpenAIRE |
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