comH, a novel gene essential for natural transformation of Helicobacter pylori
| Autor: | Jetta J. E. Bijlsma, Christina M. J. E. Vandenbroucke-Grauls, Johannes G. Kusters, Leonard C. Smeets, Sacha Y. Boomkens |
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| Rok vydání: | 2000 |
| Předmět: |
Genetics
biology Helicobacter pylori Mutant Genetic Complementation Test Genetic Vectors Genetics and Molecular Biology Bacterial genome size Sequence Analysis DNA Nitroreductases biology.organism_classification Microbiology Transformation (genetics) Plasmid Bacterial Proteins Genes Bacterial Helicobacter felis Helicobacter acinonychis Mutagenesis Site-Directed Genomic library Transformation Bacterial Molecular Biology Gene Gene Library |
| Zdroj: | Journal of bacteriology. 182(14) |
| ISSN: | 0021-9193 |
| Popis: | Helicobacter pylori is naturally competent for transformation, but the DNA uptake system of this bacterium is only partially characterized, and nothing is known about the regulation of competence in H. pylori . To identify other components involved in transformation or competence regulation in this species, we screened a mutant library for competence-deficient mutants. This resulted in the identification of a novel, Helicobacter -specific competence gene ( comH ) whose function is essential for transformation of H. pylori with chromosomal DNA fragments as well as with plasmids. Complementation of comH mutants in trans completely restored competence. Unlike other transformation genes of H. pylori , comH does not belong to a known family of orthologous genes. Moreover, no significant homologs of comH were identified in currently available databases of bacterial genome sequences. The comH gene codes for a protein with an N-terminal leader sequence and is present in both highly competent and less-efficient transforming H. pylori strains. A comH homolog was found in Helicobacter acinonychis but not in Helicobacter felis and Helicobacter mustelae. |
| Databáze: | OpenAIRE |
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