CLINICAL RESULTS OF PROPHYLACTIC INTRAVESICAL CHEMOTHERAPY ON SUPERFICIAL BLADDER CANCER USING THE DYE EXCLUSION ASSAY
| Autor: | Ken Aikawa, Yasuo Shiraiwa, Osamu Yamaguchi, Chiharu Irisawa, Masahiko Ogihara, Kiyoo Koseki, Tatsuru Hashimoto |
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| Rok vydání: | 1992 |
| Předmět: |
Adult
Male Drug medicine.medical_specialty Premedication Urology media_common.quotation_subject Microgram Urinary Bladder Antineoplastic Agents Tumor Cells Cultured Humans Cytotoxic T cell Medicine Aged media_common Aged 80 and over Carcinoma Transitional Cell Bladder cancer business.industry Mitomycin C Dye exclusion Trypan Blue Middle Aged medicine.disease Surgery Administration Intravesical Urinary Bladder Neoplasms Superficial bladder cancer Female Drug Screening Assays Antitumor Neoplasm Recurrence Local business Intravesical chemotherapy |
| Zdroj: | The Japanese Journal of Urology. 83:1828-1834 |
| ISSN: | 1884-7110 0021-5287 |
| DOI: | 10.5980/jpnjurol1989.83.1828 |
| Popis: | A chemosensitivity test was carried out on superficial bladder cancer using the dye exclusion assay for the purpose of screening chemosensitive drugs for prophylactic intravesical chemotherapy. Bladder cancer cells of each patients were incubated, in vitro, in the presence of adriamycin, (2"R)-4'-0-tetrahydropyranyladriamycin, mitomycin C, pepleomycin and 4'-epi-adriamycin (500 micrograms/ml) at 5%, CO2, 37 degrees C for 2 hours. The cytotoxic effect of the drugs was evaluated by the ratio of stained cells by trypan-blue. The most effective drug was instilled postoperatively into the bladder 3 times for the first week, and every 2 weeks during the following 14 weeks. In 18 patients followed more than 4 months the prophylactic effect was evaluated. Fifteen of the 18 patients completed the protocol, but the remaining 3 patients failed to complete to the instillation because of severe irritability of the bladder. Tumor recurrence was demonstrated in two patients. Non-recurrence rates of tumors at 12 and 24 months were 93.8% and 82.0%, respectively. These results suggested that this rapid and handy assay was useful for the purpose of screening chemosensitive drugs for the intravesical chemotherapy. |
| Databáze: | OpenAIRE |
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