Rostro-caudal maturation of glial cells in the accessory olfactory system during development: involvement in outgrowth of GnRH neurites

Autor: Pascal Vaudin, Anne Duittoz, Didier Lomet, Yves Tillet, Alain Caraty, Sarah Geller
Přispěvatelé: Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Agence Nationale pour la Recherche (ANR- NEED: Neuroendocrine Effects of Endocrine Disrupters), Région Centre (ToxEmergenCe program): Ministère de l'Enseignement Supérieur et de la Recherche, ANR-08-CESA-0011,NEED,Effet neuroendocrines de perturbateurs endocriniens, xénoestrogènes et dioxines, sur les circuits centraux de contrôle de la reproduction, notamment les systèmes GnRH(2008), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), ToxEmergenCe
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Olfactory system
Vomeronasal organ
[SDV]Life Sciences [q-bio]
olfactory ensheathing cells
souris
LHRH
Gonadotropin-Releasing Hormone
BLBP
[SCCO]Cognitive science
Mice
0302 clinical medicine
Neural Stem Cells
Cell Movement
GFAP-GFP
Neurons
General Neuroscience
Stem Cells
Neurogenesis
Peripherin
Olfactory Bulb
Cell biology
système nerveux central
cerveau
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Vomeronasal Organ
Neuroglia
hormones
hormone substitutes
and hormone antagonists
Autre (Sciences du Vivant)
endocrine system
Neurite
Neuronal Outgrowth
Mice
Transgenic
Biology
03 medical and health sciences
Organ Culture Techniques
neurone à gnrh
Neurites
Animals
système olfactif
Nasal Septum
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
Neurosciences
[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology
030104 developmental biology
nervous system
Neurons and Cognition
Forebrain
Nasal placode
cellule gliale
Olfactory ensheathing glia
030217 neurology & neurosurgery
Zdroj: European Journal of Neuroscience
European Journal of Neuroscience, Wiley, 2017, 46 (10), pp.2596-2607. ⟨10.1111/ejn.13732⟩
European Journal of Neuroscience 10 (46), 2596-2607. (2017)
ISSN: 0953-816X
1460-9568
DOI: 10.1111/ejn.13732⟩
Popis: International audience; During mammalian embryonic development, GnRH neurones differentiate from the nasal placode and migrate through the nasal septum towards the forebrain. We previously showed that a category of glial cells, the olfactory ensheathing cells (OEC), forms the microenvironment of migrating GnRH neurones. Here, to characterize the quantitative and qualitative importance of this glial, we investigated the spatiotemporal maturation of glial cells in situ and the role of maturing glia in GnRH neurones development ex vivo. More than 90% of migrating GnRH neurones were found to be associated with glial cells. There was no change in the cellular microenvironment of GnRH neurones in the regions crossed during embryonic development as glial cells formed the main microenvironment of these neurones (53.4%). However, the phenotype of OEC associated with GnRH neurones changed across regions. The OEC progenitors immunoreactive to brain lipid binding protein formed the microenvironment of migrating GnRH neurones from the vomeronasal organ to the telencephalon and were also present in the diencephalon. However, during GnRH neurone migration, maturation of OEC to [GFAP+] state (glial fibrillary acid protein) was only observed in the nasal septum. Inducing depletion of OEC in maturation, using transgenic mice expressing herpes simplex virus thymidine kinase driven by the GFAP promoter, had no impact on neurogenesis or on triggering GnRH neurones migration in nasal explant culture. Nevertheless, depletion of [GFAP+] cells decreased GnRH neurites outgrowth by 57.4%. This study suggests that specific maturation of OEC in the nasal septum plays a role in morphological differentiation of GnRH neurones.
Databáze: OpenAIRE
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