Molecular cloning and expression patterns of Cu/Zn-superoxide dismutases in developing soybean seeds
| Autor: | Chikafusa Fukazawa, Kazunari Kadokura, Van Hai Nong, Keitarou Kimura, Masaomi Arahira, Kyoko Udaka |
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| Rok vydání: | 1998 |
| Předmět: |
Molecular Sequence Data
Molecular cloning Applied Microbiology and Biotechnology Biochemistry Isozyme Analytical Chemistry Superoxide dismutase Gene Expression Regulation Plant Complementary DNA Gene expression Storage protein Northern blot Amino Acid Sequence Cloning Molecular Molecular Biology DNA Primers chemistry.chemical_classification biology Base Sequence Superoxide Dismutase Organic Chemistry food and beverages General Medicine Enzyme chemistry Seeds biology.protein Soybeans Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 62(5) |
| ISSN: | 0916-8451 |
| Popis: | Assuming that the amount of superoxide radicals generated in vivo correlates with the production of ergastic substances such as storage proteins, the coordinated response of detoxication enzymes such as superoxide dismutases is largely exploited to understand the self-defense systems of plant. Here we examined expression of the genes for superoxide dismutases during seed development of soybean. The cDNAs encoding a cytosolic copper/zinc form and an iron form of the above enzyme have been cloned and then employed as probes, separately. Northern blotting results suggested that both superoxide dismutase mRNAs are expressed at the maximum level, preceding a developmental stage when mRNA encoding glycinin, soybean 11S-storage protein, at the maximum. |
| Databáze: | OpenAIRE |
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