Photo-crosslinked GelMA/collagen membrane loaded with lysozyme as an antibacterial corneal implant
| Autor: | Peng Yuehai, Xiangjing Yang, Huang Yongrui, Jia Liu, Li Ren, Yingni Xu, Xiaomin Sun |
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| Rok vydání: | 2021 |
| Předmět: |
Staphylococcus aureus
Biocompatibility medicine.medical_treatment Biochemistry Corneal Transplantation chemistry.chemical_compound Structural Biology Cornea Cell Adhesion medicine Animals Cell adhesion Molecular Biology Cells Cultured Corneal transplantation Epithelium Corneal Membranes Artificial General Medicine Anti-Bacterial Agents Transplantation Drug Liberation Cross-Linking Reagents Membrane medicine.anatomical_structure chemistry Muramidase Collagen Rabbits Implant Lysozyme Biomedical engineering |
| Zdroj: | International Journal of Biological Macromolecules. 191:1006-1016 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2021.09.144 |
| Popis: | Corneal transplantation is an effective treatment for corneal blindness. However, it brings risk factors for the occurrence of bacterial keratitis, which can affect the repair effect and even lead to transplantation failure. The difficulty in re-epithelialization is also a main problem faced by corneal transplantation. Herein, a collagen-GelMA composite membrane containing lysozyme (CGL) was developed as an antibacterial corneal implant to fill stromal defect and support re-epithelialization. Characterizations of physicochemical properties and in vitro biocompatibility revealed that the composite membranes have proper water content, light transmittance and mechanical strength as well as good biocompatibility. Particularly, the cell adhesion force and adhesion-related genes expression were evaluated and exhibited an improvement after the addition of GelMA. Furthermore, the formed CGL membrane could continuously release lysozyme and exhibited a bactericidal rate of 96% and 64% after 2 h and 72 h, respectively. The results demonstrated that this CGL membrane has promising application in corneal repair. |
| Databáze: | OpenAIRE |
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