Central and peripheral clocks in Atlantic bluefin tuna (Thunnus thynnus, L.): Daily rhythmicity of hepatic lipid metabolism and digestive genes
| Autor: | Eloise Perkins, Aurelio Ortega, Douglas R. Tocher, Fernando de la Gándara, Matthew Sprague, Gabriel Mourente, Mónica B. Betancor, Renata Ruth |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
digestive enzyme genes
medicine.medical_specialty Acuicultura brain growth Aquatic Science liver survival 03 medical and health sciences Internal medicine lipid metabolism medicine clock genes ongrowing 14. Life underwater Circadian rhythm Centro Oceanográfico de Murcia Liver X receptor 030304 developmental biology atún rojo chronometers fish 0303 health sciences biology Lipid metabolism 04 agricultural and veterinary sciences daily Sterol regulatory element-binding protein CLOCK PER2 Endocrinology aquaculture Digestive enzyme 040102 fisheries biology.protein 0401 agriculture forestry and fisheries bluefin tuna Thunnus thynnus metabolism PER1 |
| Zdroj: | Aquaculture e-IEO. Repositorio Institucional Digital de Acceso Abierto del Instituto Español de Oceanografía instname e-IEO: Repositorio Institucional Digital de Acceso Abierto del Instituto Español de Oceanografía Instituto Español de Oceanografía |
| Popis: | Atlantic bluefin tuna (ABT; Thunnus thynnus) is a highly regarded and consumed species, but farming is still in its infancy. Currently, nothing is known about the presence of circadian rhythmicity at central or peripheral tissues, or if there are daily rhythms in expression of genes involved in lipid metabolism. In order to elucidate clock gene regulation of genes of lipid metabolism in ABT, six clock genes (bmal1, clock, cry1, cry2, per1 and per2) were sequenced and 24 h expression of these genes determined in brain and liver of fish acclimated to a light:dark (L:D) photoperiod. Additionally, the daily expression of lipid metabolism and digestive enzyme genes in ABT was also determined in liver. All six clock genes displayed rhythmicity in the brain and liver, other than cry2, which did not show an acrophase in liver. In liver, all the transcription factors analysed other than srebp1 and srebp2 displayed rhythmicity, with lxr and pparα displaying diurnal expression, whereas pparγ was highly expressed at the end of the scotophase. Some of the target genes of lxr such as elovl5 and lpl also oscillated rhythmically, with acrophases during the photophase. In contrast, only three of the eight digestive enzyme genes studied displayed rhythmicity, at different times of the day, suggesting that either ABT display different feeding periods or the digestion of some nutrients (e.g. lipids) is prioritized over others. The present study showed that clock and lipid metabolism genes displayed strong daily rhythmicity in ABT brain and liver, which could be an area of considerable interest for the establishment of efficient feeding protocols in this new aquaculture species. SI |
| Databáze: | OpenAIRE |
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