Postcolumn Radionuclide Detection of Low-Energy .beta. Emitters in Capillary Electrophoresis

Autor:	Verna Toma, Scott E. Tracht, Jonathan V. Sweedler
Rok vydání:	1994
Předmět:	Electrophoresis Detection limit Analyte Chromatography Chemistry Elution Peptide binding Sulfur Radioisotopes Tritium Beta Particles Analytical Chemistry Capillary electrophoresis Beta particle Light emission
Zdroj:	Analytical Chemistry. 66:2382-2389
ISSN:	1520-6882 0003-2700
DOI:	10.1021/ac00086a026
Popis:	A postcolumn radionuclide detection system for capillary electrophoresis (CE) is described. Eluant from an electrophoresis capillary is directed onto a peptide binding membrane that has been previously coated with a solid scintillator. The membrane is moved in a preselected pattern relative to the fixed capillary outlet during electrophoresis. Light emission from scintillation is imaged onto a charge-coupled device (CCD) using a series of 35-mm camera lenses. Detection of two low-energy beta- emitters (35S and 3H) not previously reported for capillary electrophoresis is demonstrated. The separation efficiencies are similar to those obtained with on-line UV detection. The response for 35S-labeled methionine is linear (r2 = 0.996) from 66 amol to 11 fmol. Detection limits are 88 zmol (0.03 Bq) for 32P-labeled analytes, 17 amol (0.94 Bq) for 35S-labeled analytes, and 8 fmol (8.5 Bq) for 3H-labeled analytes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d27917a8ec7afc9bbfb036f0b820dc5 https://doi.org/10.1021/ac00086a026 Zobrazit plný text záznamu