Sleeve Gastrectomy enhances glucose utilization and remodels adipose tissue independent of weight loss
| Autor: | Amir Mina, David A. Harris, Keyvan Heshmati, Alexander S. Banks, Renuka Subramaniam, Dimitrije Cabarkapa, Eric G. Sheu, Ali Tavakkoli |
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| Rok vydání: | 2019 |
| Předmět: |
Blood Glucose
Male 0301 basic medicine medicine.medical_specialty Physiology Endocrinology Diabetes and Metabolism Glucose uptake Adipose tissue 030209 endocrinology & metabolism Eating Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Downregulation and upregulation Gastrectomy Weight loss Physiology (medical) Adipocyte Internal medicine Weight Loss medicine Animals Homeostasis Glucose homeostasis Respiratory system 030304 developmental biology 2. Zero hunger 0303 health sciences Cell chemotaxis Calorimetry Indirect Glucose Tolerance Test Carbohydrate Glucose 030104 developmental biology Endocrinology Adipose Tissue chemistry Models Animal Body Composition medicine.symptom Research Article |
| Zdroj: | Am J Physiol Endocrinol Metab |
| Popis: | ObjectiveSleeve gastrectomy (SG) induces weight-loss independent improvements in glucose homeostasis by unknown mechanisms. We sought to identify the metabolic adaptations responsible for these improvements.MethodsNon-obese C57Bl6/J mice on standard chow underwent SG or sham surgery. Functional testing and indirect calorimetry were used to capture metabolic phenotypes. Tissue-specific glucose uptake was assessed by 18-FDG PET/CT and RNA sequencing was used for gene expression analysis.ResultsIn this model, SG induced durable improvements in glucose tolerance despite not causing lasting changes in weight, fat/lean mass, or food intake. Indirect calorimetry revealed post-SG animals had respiratory exchange ratios (RER) nearing 1.0 on average and had daily RER excursions above 1.0, indicating preferential glucose utilization and increased energy demand, respectively. Sham operated mice demonstrate normal RER feeding/fasting excursions. PET/CT showed increased avidity within white adipose depots. Finally, SG led to an upregulation in the transcriptional pathways involved in energy metabolism, adipocyte maturation, and adaptive and innate immune cell chemotaxis and differentiation within the visceral adipose tissue.ConclusionsSG induces a rapid, weight-loss independent shift towards glucose utilization and transcriptional remodeling of metabolic and immune pathways in visceral adipose tissue. |
| Databáze: | OpenAIRE |
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