Repeated lipopolysaccharide exposure modifies immune and sickness behaviour response in an animal model of chronic inflammation
| Autor: | S. E. Aldridge, Carmine M. Pariante, Sandrine Thuret, Cathy Fernandes, Ksenia Musaelyan, Martin Egeland, Andrea Du Preez, Patricia A. Zunszain |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides Male Necrosis Lipopolysaccharide chronic inflammation sickness behaviour adult neurogenesis hippocampus cytokine microglia Lipopolysaccharide medicine.medical_treatment Inflammation Pharmacology 03 medical and health sciences chemistry.chemical_compound Eating Mice 0302 clinical medicine Immune system Weight loss Weight Loss medicine Animals Pharmacology (medical) Illness Behavior Mice Inbred BALB C Microglia Behavior Animal Dose-Response Relationship Drug business.industry Depression Dentate gyrus Psychiatry and Mental health Disease Models Animal 030104 developmental biology medicine.anatomical_structure Cytokine chemistry Immunology Chronic Disease Cytokines medicine.symptom business 030217 neurology & neurosurgery Injections Intraperitoneal |
| Zdroj: | Musaelyan, K, Aldridge, S E, Du Preez, A, Egeland, M T L, Zunszain, P A, Pariante, C M, Thuret, S & Fernandes, C 2017, ' Repeated lipopolysaccharide exposure modifies immune and sickness behaviour response in an animal model of chronic inflammation ', Journal of Psychopharmacology, vol. 32, no. 2, pp. 236-247 . https://doi.org/10.1177/0269881117746902 |
| ISSN: | 1461-7285 |
| DOI: | 10.1177/0269881117746902 |
| Popis: | Repeated lipopolysaccharide exposure is often used in longitudinal preclinical models of depression. However, the potential phenotypic differences from acute depression-mimicking effects are rarely described. This study compared chronic lipopolysaccharide administration of doses previously used in depression research to a new mode of escalating dose injections. Adult male BALB/c mice ( n=8/group) were injected intraperitoneally with either a single 0.83 mg/kg dose, a repeated 0.1 mg/kg lipopolysaccharide dose or a dose which escalated weekly from 0.33 to 0.83 mg/kg lipopolysaccharide for six weeks. The escalating lipopolysaccharide group demonstrated most features of sickness behaviour such as weight loss and reduction in food intake every week, whilst this effect was not sustained in other groups. Moreover, only in the escalating lipopolysaccharide group did most peripheral plasma cytokines levels, measured using Luminex multiplex technology, such as interleukin-6, tumour necrosis factor α and interleukin-2 remain over three-fold elevated on the sixth week. In addition, exposure to escalating doses led to a reduction of neuroblast maturation in the dentate gyrus relevant for depression neurobiology. Therefore, this mode of injections might be useful in the studies attempting to replicate neurobiological aspects of the chronic inflammatory state observed in mood disorders. |
| Databáze: | OpenAIRE |
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