Effect of dietary n-3 polyunsaturated fatty acid rich fish oil on the endometrial prostaglandin production in the doe ( Capra hircus )
| Autor: | Narayanan Krishnaswamy, Ravi Kumar Gandham, Sanjay Kumar Singh, Harendra Kumar, A. S. Mahla, Amit Kumar Singh, Ravjibhai Karshanbhai Chaudhari, Gyanendra Singh, A.M. Pawde, Mihir Sarkar |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Physiology Prostaglandin Endometrium Biochemistry Andrology 03 medical and health sciences chemistry.chemical_compound Dietary Fats Unsaturated Fatty Acids Omega-3 medicine Animals Pharmacology Estrous cycle chemistry.chemical_classification Chemistry Goats 0402 animal and dairy science 04 agricultural and veterinary sciences Cell Biology Fish oil 040201 dairy & animal science Interferon tau Phospholipases A2 030104 developmental biology medicine.anatomical_structure Blood chemistry Cyclooxygenase 2 Hydroxyprostaglandin Dehydrogenases Prostaglandins Female Explant culture Polyunsaturated fatty acid |
| Zdroj: | Prostaglandins & Other Lipid Mediators. 135:27-35 |
| ISSN: | 1098-8823 |
| Popis: | Recently, we showed that dietary supplementation of n-3 PUFA rich fish oil (FO) decreased the metabolites of serum prostaglandin (PG) F2α and E2 during the window of pregnancy recognition in the doe. In this study, we investigated its effect on the changes on endometrial PG production in vitro. Cycling does (n = 12) of Rohilkhand region were divided into two equal groups and fed a concentrate diet supplemented with either FO containing 26% n-3 PUFA (TRT; n = 6) or palm oil (CON; n = 6) @ 0.6 mL/kg body weight for 57 days. Estrus was synchronized by two injections of PGF2α analogue viz, on day 25 and 36 of supplementation and laparo-hysterotomy was performed to obtain endometrial tissue on day 16 of the synchronized estrus. Endometrial explant culture was done using a defined medium.The basal PG production was assayed at 6 and 12 h. Endometrial explant was stimulated with oxytocin (OXT) and/or recombinant ovine interferon tau (roIFN-τ) and PGs were assayed at 3 and 12 h post-treatment. The relative expression of genes related to PG metabolism in the endometrium was done by Quantitative Real Time PCR technique (qRT-PCR). There was a significant (P 0.05) effect on the PGF2α and PGE2 production in the TRT group. Similarly, the PG production in the OXT and roIFN-τ was comparable with the control in TRT. Expression of mRNA for cyclooxygenase-2 (COX-2), cytosolic phospholipase A2 (cPLA2) and PGF synthase (PGFS) was lower (P |
| Databáze: | OpenAIRE |
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