Serum paraoxonase 1 activity in cats: analytical validation, reference intervals, and correlation with serum amyloid A and alpha-1-acid glycoprotein
| Autor: | Sara Meazzi, Saverio Paltrinieri, Gabriele Rossi, Alessia Giordano |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty 040301 veterinary sciences Orosomucoid Cat Diseases 0403 veterinary science 03 medical and health sciences Lipid oxidation Reference Values Internal medicine medicine Animals Humans Full Scientific Reports Serum amyloid A 030304 developmental biology Serum Amyloid A Protein 0303 health sciences CATS General Veterinary biology Aryldialkylphosphatase Chemistry Acute-phase protein Paraoxonase Reproducibility of Results 04 agricultural and veterinary sciences medicine.disease PON1 Hemolysis Endocrinology Cats biology.protein Female Biomarkers |
| Zdroj: | J Vet Diagn Invest |
| ISSN: | 1943-4936 1040-6387 |
| Popis: | Paraoxonase 1 (PON1) is an inflammation marker associated with lipid oxidation and is used as a diagnostic marker in people. There is no information about the suitable substrate and analytic performance in cats, or its biological behavior compared with other inflammation markers. Our aims were to validate a paraoxon-based method to measure PON1 activity in feline serum, to assess stability of PON1 under different storage conditions and the impact of interfering elements, to determine a reference interval (RI) for healthy cats, and to correlate PON1 activity with 2 major acute-phase proteins. Intra- and inter-assay precision, accuracy, and RI were assessed using fresh serum. The same specimens were stored at room temperature, refrigerated, or frozen, and retested at defined intervals. Hemolysis, lipemia, and icterus were simulated to study interferences. PON1 results were compared to serum amyloid A (SAA) and alpha-1-acid glycoprotein (AGP) results. Analytical validation yielded precise and accurate results. PON1 activity is stable for up to 24 h at room temperature and up to 48 h at 4°C. Freezing at −20°C results in an increase after 72 h, with return to baseline values after 1 wk, that again increases after 6 mo. Only hyperlipemia interfered with PON1 activity. The RI based on 71 healthy cats was 58–154 U/L. PON1 activity was negatively correlated with AGP, but not with SAA. Serum PON1 activity can be measured accurately in cats, and it acts as a negative acute-phase protein. |
| Databáze: | OpenAIRE |
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