Diesel particles induce nitric oxide production in murine alveolar macrophages and rat airways
| Autor: | Hannu Komulainen, Raimo O. Salonen, Marjo Ruotsalainen, Arja J Hälinen, Maija-Riitta Hirvonen |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Pharmacology
medicine.diagnostic_test Chemistry Health Toxicology and Mutagenesis medicine.medical_treatment General Medicine Toxicology Pulmonary edema medicine.disease Nitric oxide chemistry.chemical_compound Bronchoalveolar lavage medicine.anatomical_structure Cytokine Immunology medicine Macrophage Tumor necrosis factor alpha Respiratory system Respiratory tract |
| Zdroj: | Environmental Toxicology and Pharmacology. 7:11-18 |
| ISSN: | 1382-6689 |
| DOI: | 10.1016/s1382-6689(98)00044-1 |
| Popis: | The acute adverse health effects among respiratory and cardiovascular patients have been associated with particulate air pollution, containing diesel particles (DP). The mechanisms of these effects are unknown, but they may involve inflammation. We investigated the effects of DP (30-3000 μg/10(6) cells) on cell viability and production of nitric oxide (NO), interleukin-1 (IL-1), interleukin-6 (IL-6), interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) in murine RAW 264.7 macrophage cultures in vitro. DP caused a dose- and time-dependent NO-production and was cytotoxic in murine RAW 264.7 macrophages. Cytotoxicity preceded the increases in NO production. DP had minimal effects on cytokine production. A single intratracheal instillation of DP 1 and 5 mg/rat increased NO production and protein concentration in bronchoalveolar lavage fluid, and caused pulmonary edema and hemorrhage. The present results indicate that DP can induce both NO production and cytotoxicity in the lower respiratory tract, which may contribute to the short-term adverse respiratory effects of these particles. |
| Databáze: | OpenAIRE |
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