Impact of after-treatment devices and biofuels on diesel exhausts genotoxicity in A549 cells exposed at air-liquid interface
| Autor: | E. Estace, Stéphanie Lagadu, François Sichel, Cécile Corbière, David Preterre, Véronique André, Didier Pottier, Ivannah Pottier, Frédéric Dionnet, Christelle Monteil, K. Blanchard, C. Logie, Veronika Keravec, Claire Barraud, Jean-Paul Morin |
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| Přispěvatelé: | Aliments Bioprocédés Toxicologie Environnements (ABTE), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Centre d'Etude et de Recherche Technologique en Aérothermique et Moteurs [Saint Etienne du Rouvray] (CERTAM), Université de Rouen Normandie (UNIROUEN), Centre Régional de Lutte contre le Cancer François Baclesse [Caen] (UNICANCER/CRLC), UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU) |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
[SDV.CAN]Life Sciences [q-bio]/Cancer 010501 environmental sciences Toxicology medicine.disease_cause complex mixtures 01 natural sciences 03 medical and health sciences Diesel fuel chemistry.chemical_compound Toxicity Tests medicine Humans After-treatment devices 0105 earth and related environmental sciences Vehicle Emissions Biodiesel Air Pollutants Diesel particulate filter biology Chemistry Mutagenicity Tests Superoxide Dismutase General Medicine Glutathione respiratory system Catalase Air-liquid interface respiratory tract diseases Oxidative Stress 030104 developmental biology Biofuel A549 Cells Environmental chemistry Biofuels biology.protein Genotoxicity Reactive Oxygen Species Diesel exhausts human activities Oxidative stress DNA Damage Mutagens |
| Zdroj: | Toxicology in Vitro Toxicology in Vitro, Elsevier, 2017, 45 (Part 3), pp.426-433. ⟨10.1016/j.tiv.2017.04.025⟩ |
| ISSN: | 1879-3177 0887-2333 |
| Popis: | International audience; Using an air-liquid interface (ALI) device in dynamic conditions, we evaluated the efficiency of fuel after-treatment strategies (diesel oxidation catalysis, DOC, and diesel particulate filter, DPF, devices) and the impact of 7% and 30% rapeseed methyl esters (RME) blending on oxidative stress and genotoxicity induced in A549 lung cells after 3 h exposure to whole Diesel exhausts. Oxidative stress was studied using assays of ROS production, glutathione level, catalase and superoxide-dismutase (SOD) activities. No oxidative stress and no clear differences on cytotoxicity patterns between biodiesel and standard Diesel exhausts were found. A weak but significant genotoxicity (8-oxodGuo adducts) and, for standard Diesel only, a DNA damage response (DDR) as evidenced by ƔH2AX foci, remained after DOC + DPF flowing. All together, these data could contribute to the improvement of the after treatment strategies and to health risk assessment of current diesel exhausts. |
| Databáze: | OpenAIRE |
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