Phenotypic Screening of Small Molecule Libraries by High Throughput Cell Imaging
| Autor: | Y. Feng, Timothy J. Mitchison, Tomas Kirchhausen, Justin C. Yarrow, Zachary E. Perlman |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Cell physiology
Phenotypic screening Organic Chemistry Cell Small Molecule Libraries Cell migration General Medicine Biology Computer Science Applications Cell biology Eukaryotic Cells Phenotype medicine.anatomical_structure Microscopy Fluorescence Drug Discovery Image Processing Computer-Assisted medicine Animals Combinatorial Chemistry Techniques Humans Target protein Mitosis Secretory pathway |
| Zdroj: | Combinatorial Chemistry & High Throughput Screening. 6:279-286 |
| ISSN: | 1386-2073 |
| Popis: | We have developed high throughput fluorescence cell imaging methods to screen chemical libraries for compounds with effects on diverse aspects of cell physiology. We describe screens for compounds that arrest cells in mitosis, that block cell migration, and that block the secretory pathway. Each of these screens yielded specific inhibitors for research use, and the mitosis screen identified Eg5 as a potential target protein for cancer chemotherapy. Cell imaging provides a large amount of information from primary screening data that can be used to distinguish compounds with different effects on cells, and together with automated analysis, to quantitate compound effects. |
| Databáze: | OpenAIRE |
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