A Null Mutation in Basigin, an Immunoglobulin Superfamily Member, Indicates Its Important Roles in Peri-implantation Development and Spermatogenesis
| Autor: | Masahide Takahashi, Tadashi Kaname, Hisako Muramatsu, Osamu Taguchi, Kenji Kadomatsu, Teruo Miyauchi, Naohiko Kuno, Takashi Muramatsu, Takao Senda, Ken Ichi Yamamura, Qi-Wen Fan, Tadahiko Igakura, Kimiyoshi Arimura, Yoshiro Toyama, Shigeki Yuasa |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Mutant Biology Avian Proteins Mice Antigens CD Antigens Neoplasm Animals Embryo Implantation Spermatogenesis Metaphase Molecular Biology Mice Knockout Membrane Glycoproteins Gene targeting Embryo Blood Proteins Cell Biology Null allele Mice Mutant Strains Cell biology Basigin Immunology Antigens Surface Mutation Immunoglobulin superfamily Female Developmental Biology |
| Zdroj: | Developmental Biology. 194(2):152-165 |
| ISSN: | 0012-1606 |
| DOI: | 10.1006/dbio.1997.8819 |
| Popis: | Basigin is a highly glycosylated transmembrane protein with two immunoglobulin-like domains. We generated mutant mice lacking the basigin gene (Bsg) by gene targeting. Bsg (-/-) embryos developed normally during preimplantation stages. However, the majority of Bsg (-/-) embryos died around the time of implantation. At this time, basigin mRNA was strongly expressed in the trophectoderm, embryo proper, and uterine endometrium of Bsg (+/+) mice. These results suggest that basigin is involved in intercellular recognition during implantation. Embryos which survived the critical period yielded Bsg (-/-) mutant mice. Half of the mutant mice died before 1 month after birth, due to interstitial pneumonia. The surviving adult mutant mice were small and sterile. Spermatogenesis was arrested in the mutant mice. Most of the spermatocytes in the Bsg (-/-) mouse were arrested and degenerated at the metaphase of the first meiosis, and only a small number differentiated to step 1 spermatids. In the female mutants, the ovaries and genital tract were morphologically normal, and the defect was probably in the capability of implantation of the uterus. In conclusion, basigin is an important cell-surface molecule involved in early embryogenesis and reproduction. |
| Databáze: | OpenAIRE |
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