Erratum: Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses. Corrigendum
| Autor: | Filipe R. N. C. Maia, Jason E. Koglin, W. Henry Benner, Kerstin Mühlig, Dirk Hasse, Asawari D. Rath, Nicusor Timneanu, Martin Svenda, Jakob Andreasson, Salah Awel, Alberto Pietrini, Gijs van der Schot, Jonas A. Sellberg, Johan Bielecki, M. Marvin Seibert, Garth J. Williams, Inger Andersson, Anton Barty, N. Duane Loh, Richard A. Kirian, Daniel Westphal, Carl Nettelblad, Benedikt J. Daurer, Max F. Hantke, Gunilla H. Carlsson, Tomas Ekeberg, Kenta Okamoto, Sébastien Boutet, Daniel S. D. Larsson, Max O. Wiedorn, Alessandro Zani, Janos Hajdu |
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| Rok vydání: | 2019 |
| Předmět: |
Materials science
business.industry flash X-ray imaging X-ray Free-electron laser General Chemistry virus Addenda and Errata Condensed Matter Physics Omono River virus Biochemistry Research Papers X-ray diffraction Optics free-electron laser Femtosecond X-ray crystallography diffraction before destruction General Materials Science lcsh:Q business lcsh:Science OmRV |
| Zdroj: | IUCrJ IUCrJ, Vol 6, Iss 3, Pp 500-500 (2019) |
| ISSN: | 2052-2525 |
| Popis: | Facilitating the very short and intense pulses from an X-ray laser for the purpose of imaging small bioparticles carries the potential for structure determination at atomic resolution without the need for crystallization. In this study, experimental strategies for this idea are explored based on data collected at the Linac Coherent Light Source from 40 nm virus particles injected into a hard X-ray beam. This study explores the capabilities of the Coherent X-ray Imaging Instrument at the Linac Coherent Light Source to image small biological samples. The weak signal from small samples puts a significant demand on the experiment. Aerosolized Omono River virus particles of ∼40 nm in diameter were injected into the submicrometre X-ray focus at a reduced pressure. Diffraction patterns were recorded on two area detectors. The statistical nature of the measurements from many individual particles provided information about the intensity profile of the X-ray beam, phase variations in the wavefront and the size distribution of the injected particles. The results point to a wider than expected size distribution (from ∼35 to ∼300 nm in diameter). This is likely to be owing to nonvolatile contaminants from larger droplets during aerosolization and droplet evaporation. The results suggest that the concentration of nonvolatile contaminants and the ratio between the volumes of the initial droplet and the sample particles is critical in such studies. The maximum beam intensity in the focus was found to be 1.9 × 1012 photons per µm2 per pulse. The full-width of the focus at half-maximum was estimated to be 500 nm (assuming 20% beamline transmission), and this width is larger than expected. Under these conditions, the diffraction signal from a sample-sized particle remained above the average background to a resolution of 4.25 nm. The results suggest that reducing the size of the initial droplets during aerosolization is necessary to bring small particles into the scope of detailed structural studies with X-ray lasers. |
| Databáze: | OpenAIRE |
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