Tissue superoxide dismutase (SOD) activity and immunohistochemical staining in acute appendicitis: Correlation with degree of inflammation
| Autor: | Taiju Hashimoto, Hirokazu Kawase, Akira Satomi, Shigeki Takahashi, Moriyuki Matsuki, Hideaki Murai, Saburo Murakami, Takao Morita, Masaru Sonoda |
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| Rok vydání: | 1996 |
| Předmět: |
Adult
medicine.medical_specialty Pathology Adolescent Thiobarbituric acid Inflammation Sensitivity and Specificity Thiobarbituric Acid Reactive Substances Diagnosis Differential Superoxide dismutase chemistry.chemical_compound Reference Values Culture Techniques Internal medicine medicine TBARS Humans Child Aged Aged 80 and over biology Superoxide Dismutase Chemistry Gastroenterology Middle Aged Hepatology Appendicitis medicine.disease Immunohistochemistry Appendix medicine.anatomical_structure Child Preschool Acute Disease Disease Progression biology.protein medicine.symptom |
| Zdroj: | Journal of Gastroenterology. 31:639-645 |
| ISSN: | 1435-5922 0944-1174 |
| Popis: | The mechanism of progression of appendicitis has not been clarified. We examined tissue superoxide dismutase (SOD) activity, thiobarbituric acid reactive substance (TBARS), and the localization of Cu, Zn-SOD in 56 inflamed appendices in relation to histopathological classification. There was a significant difference in SOD activity between catarrhal appendix and phlegmonous and gangrenous appendix (2.3 +/- 0.1 vs 5.0 +/- 0.2 and 4.6 +/- 0.6 units/mg protein, respectively P < 0.05). TBARS value was highest in gangrenous appendix, being significantly different from the levels in the other two types (0.47 +/- 0.04 vs 0.19 +/- 0.01 n mol/mg protein, in catarrhal and 0.20 +/- 0.02, in phlegmonous appendix P < 0.05). Positive staining for Cu, Zn-SOD was demonstrated in 64% of catarrhal appendices, 96% of phlegmonous appendices, and 75% of gangrenous appendices, and intense positive staining was recognized in 9%, 28%, and 40% of these appendices, respectively. These results indicated that active oxygen influences the degree of inflammation in phlegmonous and gangrenous appendicitis. Gangrenous appendicitis and the other two types of appendicitis seemed to be different entities. |
| Databáze: | OpenAIRE |
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