Injectable Gel Form of a Decellularized Bladder Induces Adipose-Derived Stem Cell Differentiation into Smooth Muscle Cells In Vitro

Autor: Daria Zaytseva-Zotova, Eric Lopez-Mocholi, Ángel Serrano-Aroca, Berit L. Strand, Álvaro Briz-Redón, Victoria Moreno-Manzano
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Detrusor muscle
Swine
Adipose tissue
scaffold
Extracellular matrix
lcsh:Chemistry
0302 clinical medicine
Tissue engineering
lcsh:QH301-705.5
Spectroscopy
viscoelasticity
Decellularization
Urinary bladder
Chemistry
Stem Cells
Hydrogels
gel
extracellular matrix
rheology
pepsin
adipose-derived stem cells
smooth muscle cells
cell differentiation
Matrigel
General Medicine
Computer Science Applications
Cell biology
medicine.anatomical_structure
Adipose Tissue
030220 oncology & carcinogenesis
Female
Rabbits
Stem cell
Myocytes
Smooth Muscle
Urinary Bladder
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
medicine
Animals
Humans
Physical and Theoretical Chemistry
Molecular Biology
Tissue Engineering
Organic Chemistry
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Zdroj: International Journal of Molecular Sciences, Vol 21, Iss 8608, p 8608 (2020)
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
instname
International Journal of Molecular Sciences
Volume 21
Issue 22
r-CIPF: Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
Centro de Investigación Principe Felipe (CIPF)
ISSN: 1661-6596
1422-0067
Popis: Biologic scaffolds composed of extracellular matrix components have been proposed to repair and reconstruct a variety of tissues in clinical and pre-clinical studies. Injectable gels can fill and conform any three-dimensional shape and can be delivered to sites of interest by minimally invasive techniques. In this study, a biological gel was produced from a decellularized porcine urinary bladder by enzymatic digestion with pepsin. The enzymatic digestion was confirmed by visual inspection after dissolution in phosphate-buffered saline solution and Fourier-transform infrared spectroscopy. The rheological and biological properties of the gel were characterized and compared to those of the MatrigelTM chosen as a reference material. The storage modulus G&rsquo
reached 19.4 ±
3.7 Pa for the 30 mg/mL digested decellularized bladder gels after ca. 3 h at 37 °
C. The results show that the gel formed of the porcine urinary bladder favored the spontaneous differentiation of human and rabbit adipose-derived stem cells in vitro into smooth muscle cells to the detriment of cell proliferation. The results support the potential of the developed injectable gel for tissue engineering applications to reconstruct for instance the detrusor muscle part of the human urinary bladder.
Databáze: OpenAIRE
Externí odkaz: