Mechanism and pathway of penicillopepsin-catalyzed transpeptidation and evidence for noncovalent trapping of amino acid and peptide intermediates
| Autor: | Theo Hofmann, H Pang, Annie Cunningham, Max Blum |
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| Rok vydání: | 1991 |
| Předmět: |
Binding Sites
biology Hydrogen bond Chemistry Stereochemistry Active site Substrate (chemistry) Cell Biology Reaction intermediate Cleavage (embryo) Biochemistry Catalysis Mass Spectrometry nervous system Covalent bond Penicillopepsin Peptidyl Transferases polycyclic compounds biology.protein Peptide bond Aspartic Acid Endopeptidases Amino Acids Peptides Molecular Biology Chromatography High Pressure Liquid |
| Zdroj: | The Journal of biological chemistry. 266(15) |
| ISSN: | 0021-9258 |
| Popis: | Penicillopepsin acting on Nph-Ala2-amide (where Nph = p-nitrophenylalanyl) catalyzes a transpeptidation reaction which leads to the formation of Nph2-Ala2-amide, which arises from condensation of the substrate with enzyme-bound Nph, as the first product released from the enzyme. This is followed by a stage during which Nph3 and Ala2-amide are the major products. A small amount of Nph4 is also formed during this time. Nph and Nph2, formed during the reactions, are tightly, but probably not covalently, bound to the enzyme. They appear as free products only as a result of the cleavage of Nph3 and Nph4 and after most of the substrate Nph-Ala2-amide has been used up. They act as acceptors for the substrate and for Nph2-Ala2-amide. Nph3-Ala2-amide, formed by condensation of Nph-Ala2-amide or of Nph2-Ala2-amide with enzyme-bound Nph2 or Nph, respectively, is also released but is cleaved rapidly to give Nph3 and Ala2-amide. Incorporation of 18O from [18O]water into the carbonyl oxygens of the products is extensive and shows that release of the intermediates is slower than peptide bond cleavage and peptide bond formation. Hence the rate-limiting step in these reactions is product release. No 18O is incorporated into the initial substrate. We propose that Nph and Nph2 as intermediates are held in the active site by hydrogen bonds and by two strong electrostatic interactions. |
| Databáze: | OpenAIRE |
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