Simple and Easy Synthesis of γ-Amido-dNTPs in Water and Their Polymerase Reaction Properties
| Autor: | Ryo Miyahara, Takato Sakurada, Yoshiya Kikukawa, Yosuke Taniguchi, Yusuke Nagata, Shigeki Sasaki, Ryoji Kawazoe |
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| Rok vydání: | 2021 |
| Předmět: |
biology
Nucleotides Chemistry DNA polymerase Water DNA-Directed DNA Polymerase General Chemistry General Medicine Combinatorial chemistry Nucleobase Carbodiimides Kinetics chemistry.chemical_compound Solubility Ammonia Polyphosphates One pot reaction Drug Discovery biology.protein Selectivity Nucleoside Chromatography High Pressure Liquid Polymerase Carbodiimide |
| Zdroj: | Chemical and Pharmaceutical Bulletin. 69:1061-1066 |
| ISSN: | 1347-5223 0009-2363 |
| DOI: | 10.1248/cpb.c21-00497 |
| Popis: | γ-Amido-modified 2'-deoxynucleoside triphosphates (dNTPs) and nucleoside triphosphates (NTPs) are becoming increasingly important as biological tools. We herein describe the simple and easy synthesis of γ-amido-dNTPs and -NTPs from commercially available corresponding dNTPs and NTPs in a one-pot reaction using water-soluble carbodiimide and ammonia solution. We examined the effects of synthesized γ-amido-dNTPs on the DNA polymerase reaction. The results obtained showed the incorporation of these derivatives into the DNA primer while maintaining nucleobase selectivity; however, their incorporation efficiency by DNA polymerase was lower than that of dNTP. This is the first study to demonstrate the successful synthesis of four sets of γ-amido-dNTPs and clarify their properties. |
| Databáze: | OpenAIRE |
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