Rab11 is an apically located small GTP-binding protein in epithelial tissues
| Autor: | James R. Goldenring, Hilary D. Vaughan, Jennifer Navarre, Wendy Hawkins, Jenetta Smith, Patricia L. Cameron |
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| Rok vydání: | 1996 |
| Předmět: |
Pathology
medicine.medical_specialty Colon Physiology Liver cytology Enterocyte Blotting Western Molecular Sequence Data Immunocytochemistry Biology Kidney Bone canaliculus Epithelium Esophagus Parietal Cells Gastric GTP-Binding Proteins Physiology (medical) Pyloric Antrum medicine Animals Humans Gastric Fundus Intestinal Mucosa Pancreas DNA Primers Skin Parietal cell Mucous Membrane Base Sequence Sequence Homology Amino Acid Hepatology Gastroenterology Antibodies Monoclonal Epithelial Cells Oligonucleotides Antisense Apical membrane Immunohistochemistry Recombinant Proteins Staining medicine.anatomical_structure Liver Gastric Mucosa Organ Specificity rab GTP-Binding Proteins Rabbits |
| Zdroj: | American Journal of Physiology-Gastrointestinal and Liver Physiology. 270:G515-G525 |
| ISSN: | 1522-1547 0193-1857 |
| Popis: | Rab proteins are involved in many aspects of dynamic vesicle processing within eukaryotic cells. We have previously identified Rab11 in gastric parietal cell tubulovesicle membranes. We have produced a monoclonal antibody that is specific for Rab11. In all rabbit tissues examined, Rab11 immunoreactivity was highly enriched in epithelial cells. In the gastric fundus, parietal cells were stained in a pattern consistent with localization on tubulovesicles. Surface mucous cells of both the fundus and antrum demonstrated punctate subapical staining. Ileal and proximal colonic enterocyte labeling was observed deep to the brush borders. In the distal colon, staining was observed in the apical regions of mid-crypt cells. In skin and esophagus, punctate immunoreactivity was present in the medial layers of the squamous epithelia. Prominent Rab11 immunoreactivity was present in hepatocytes deep to the bile canaliculi. Punctate subapical staining was observed in pancreatic acinar cells. Apical staining was also observed in collecting duct cells and in the glandular cells of the prostate. These results indicate the Rab11 is expressed in apical vesicular populations in discrete epithelial cell populations. |
| Databáze: | OpenAIRE |
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