Identification of Potential Diagnostic Markers among Burkholderia cenocepacia and B. multivorans Supernatants
| Autor: | Sierrah M. Grigsby, Tiffany M. Mott, Michael Soler, Gregory C. Whitlock, Ronald Medley |
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| Rok vydání: | 2010 |
| Předmět: |
Microbiology (medical)
biology Burkholderia cenocepacia Burkholderia cepacia complex Burkholderia multivorans Bacteriology biology.organism_classification medicine.disease Phenotype Cystic fibrosis Bacterial Typing Techniques Culture Media Microbiology Burkholderia Antibiotic resistance Bacterial Proteins Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization medicine Humans Electrophoresis Gel Two-Dimensional Bacteria |
| Zdroj: | Journal of Clinical Microbiology. 48:4186-4192 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Patients with cystic fibrosis (CF) are susceptible to chronic respiratory infections with a number of bacterial pathogens. Among them, the Burkholderia cepacia complex (Bcc) bacteria, consisting of nine related species, have emerged as problematic CF pathogens due to their antibiotic resistance, incidence of nosocomial infection, and person-to-person transmission. Bcc organisms present the clinical microbiologist with a diagnostic dilemma due to the lack of phenotypic biochemical or growth-related characterization tests that reliably distinguish among these organisms. The complex taxonomy of the Bcc species colonizing the CF respiratory tract makes accurate identification problematic. Despite the clinical implications of Bcc identification, a clinical laboratory differentiation of species within the Bcc is lacking. Additionally, no commercial assays are available to further identify the Bcc species. In the current study, secretory proteins present in the cultured supernatants of Burkholderia cenocepacia and Burkholderia multivorans were analyzed by two-dimensional gel electrophoresis (2-DE), followed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). To assess differential expression, protein spots of B. cenocepacia and B. multivorans that were unique or displayed different intensities were chosen for MALDI-TOF MS analysis. In total, 341 protein spots were detected, of which 23 were unique to each species, demonstrating that potential diagnostic candidates between these two members of the Bcc exist. |
| Databáze: | OpenAIRE |
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