The production of mammalian trematode infective stages by the snail Galba truncatula
| Autor: | Philippe Vignoles, Jacques Cabaret, Gilles Dreyfuss, Daniel Rondelaud, Roselyne Mouzet |
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| Přispěvatelé: | Université de Limoges (UNILIM) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Larva
Veterinary medicine biology Pasteur pipette Petri dish fungi General Medicine Snail Fasciola hepatica Paramphistomum biology.organism_classification Paramphistomatidae law.invention law Acanthaceae biology.animal Botany parasitic diseases Animals Animal Science and Zoology Parasitology [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie Galba truncatula |
| Zdroj: | Journal of Helminthology Journal of Helminthology, Cambridge University Press (CUP), 2012, 88 (1), pp.1-7. ⟨10.1017/S0022149X1200082X⟩ |
| ISSN: | 0022-149X 1475-2697 |
| DOI: | 10.1017/S0022149X1200082X⟩ |
| Popis: | Several experiments on the breeding of trematode-infected Galba truncatula for obtaining and packaging Fasciola hepatica and Paramphistomum daubneyi metacercariae were carried out to determine the more convenient methods to use for commercial production of these infective stages. Compared to the breeding of infected snails in aquaterraria, the use of 14-cm Petri dishes allowed a greater prevalence of snail infection and a higher number of metacercariae. The production of these larvae was still 2.3–3.4 times greater if infected snails were dissected during the patent period. The aspiration of these metacercariae at the extremity of a Pasteur pipette significantly shortens the time necessary for their transfer from Petri dishes to Eppendorf tubes. Using 14-cm Petri dishes, snail dissection and metacercarial aspiration for their transfer strongly reduce the cost price for metacercarial production of the trematodes Fasciola hepatica and Paramphistomum daubneyi. |
| Databáze: | OpenAIRE |
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